G9043

抗 GRP78/BiP (ET-21) 兔抗

Name: 抗 GRP78/BiP (ET-21) 兔抗
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

别名:

抗 78-kDa 葡萄糖调节蛋白, 抗免疫球蛋白重链结合蛋白

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01865530

主要文件

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产品名称

抗 GRP78/BiP (ET-21) 兔抗, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 78 kDa

species reactivity

mouse, chicken, Xenopus, hamster, human, rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol/acetone fixed cultured mouse fibroblast NIH3T3 cell line
immunohistochemistry: 1:1,000 using human cerebral cortex and human thyroid gland tissue sections
western blot: 1:3,000 using whole cell extract of human epitheloid carcinoma HeLa cell line

UniProt accession no.

P11021

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HSPA5(3309)
mouse ... Hspa5(14828)
rat ... Hspa5(25617)

Application

兔抗 GRP78/BiP (ET-21) 抗体适合作为一抗：

用于冷冻心脏组织的免疫荧光染色，以检测心肌细胞中是否发生内质网应激信号激活
用于人大脑皮层石蜡包埋切片和人甲状腺组织切片的免疫组织化学。
通过斑马鱼 WT AB 品系 ( Danio rerio ) 蛋白提取物的免疫印迹检测 Bip
以 1:1000 的工作稀释度检测雄激素阻断治疗后前列腺癌细胞提取液中的 GRP78

还适用于使用人上皮样癌 HeLa 全细胞提取物 1:3000 工作稀释液的免疫印迹和使用小鼠成纤维细胞 NIH3T3 细胞 1:1000 工作稀释液的免疫细胞化学。

Biochem/physiol Actions

GRP78 / BiP水平在阿尔茨海默′病患者大脑中升高，研究发现GRP78 / BiP表达的降低与早老素1（PS-1）的错义突变（missense mutation）有关。

GRP78（78 kDa 葡萄糖调节蛋白）是维持细胞活力所需的热休克蛋白 Hsp70 成员。它是一种位于内质网 (ER) 的Ca²⁺-结合分子伴侣。在新合的成膜结合或分泌蛋白的正确糖基化、折叠和组装中，它具有关键作用。该蛋白还有助于保留不正确折叠的突变或存在缺陷的蛋白质，并防止此类蛋白质从细胞质易位至内质网室。该蛋白质可被氧化应激、化学毒性、糖基化和缺氧等条件诱导。它在维持细胞稳态和预防细胞凋亡中具有至关重要的作用，并可作为低血糖的生物标志物。对于暴露于谷氨酸外毒性和氧化应激的神经元，它可提供神经保护功能。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000510560

0000510564

0000279003

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Enhanced binding to the molecular chaperone BiP slows thyroglobulin export from the endoplasmic reticulum.

Z Muresan et al.

Molecular endocrinology (Baltimore, Md.), 12(3), 458-467 (1998-03-26)

To examine how binding of BiP (a molecular chaperone of the hsp70 family that resides in the endoplasmic reticulum) influences the conformational maturation of thyroglobulin (Tg, the precursor for thyroid hormone synthesis), we have developed a system of recombinant Tg

Sec61p and BiP directly facilitate polypeptide translocation into the ER.

S L Sanders et al.

Cell, 69(2), 353-365 (1992-04-17)

Secretory proteins are segregated from cytosolic proteins by their translocation into the endoplasmic reticulum (ER). A modified secretory protein trapped during translocation across the ER membrane can be crosslinked to two previously identified proteins, Sec61p and BiP (Kar2p). The dependence

Transactivation of the grp78 promoter by Ca2+ depletion. A comparative analysis with A23187 and the endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin.

W W Li et al.

The Journal of biological chemistry, 268(16), 12003-12009 (1993-06-05)

The calcium ionophore A23187 has been shown to induce the expression of a set of glucose-regulated protein (GRP) genes through the depletion of Ca2+ from the intracellular Ca2+ stores. Here we demonstrate that thapsigargin, which inhibits specifically the endoplasmic reticulum

Induction of the mammalian GRP78/BiP gene by Ca2+ depletion and formation of aberrant proteins: activation of the conserved stress-inducible grp core promoter element by the human nuclear factor YY1.

W W Li et al.

Molecular and cellular biology, 17(1), 54-60 (1997-01-01)

Previously, we have identified a constitutive nuclear factor, p70CORE, from HeLa cell nuclear extract which interacts specifically with the stress-inducible change region (SICR) of the grp78 promoter. Here we report that p70CORE is identical to YY1, a member of the

Glucose-regulated stresses induce resistance to camptothecin in human cancer cells.

A Tomida et al.

International journal of cancer, 68(3), 391-396 (1996-11-04)

The glucose-regulated stress response in mammalian cells is characterized by the increased synthesis of glucose-regulated proteins (GRPs). In this study, we found that GRP-inducing conditions in culture led to induction of resistance to the topoisomerase I-targeted drug camptothecin in human

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Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

细胞器分离

亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。

抗 GRP78/BiP (ET-21) 兔抗

IgG fraction of antiserum, buffered aqueous solution

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

enhanced validation

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

相关类别

说明

Application

Biochem/physiol Actions

Disclaimer

Physical form

安全信息

存储类别

wgk

flash_point_f

flash_point_c

法规信息

文件

分析证书(COA)

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