产品名称
抗 组蛋白 H3 兔抗, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 17 kDa
species reactivity
chicken, mouse, plant, Drosophila, rat, human, Xenopus
packaging
antibody small pack of 25 μL
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells
western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line
western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)
Application
Biochem/physiol Actions
Disclaimer
General description
H3 的磷酸化主要发生在Ser10和Ser28,对应于一小段高度乙酰化的 H3,主要由有丝分裂和应激刺激引起。Ser10磷酸化是对于有丝分裂起始很重要的事件之一。与有丝分裂和减数分裂期间的染色体浓缩关系密切。组蛋白甲基化与乙酰化一样,是一个复杂的动态过程,涉及许多过程,包括转录调节、染色质浓缩、有丝分裂和异染色质组装。组蛋白 H3、Lys4、Lys9 和 Lys27 N 端尾结构域中的保守赖氨酸残基是首选的甲基化位点。组蛋白赖氨酸残基可在不同的异染色质子域处被单甲基化、二甲基化和三甲基化,从而进一步增加染色质结构调节的复杂性。
Immunogen
Physical form
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
cells
相关内容
Datasheet
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