Merck
CN

H6908

Sigma-Aldrich

抗-HA 兔抗

affinity isolated antibody, buffered aqueous solution

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别名:
HA 抗体 - 产自兔中的抗-HA抗体, Ha抗体, Sigma Ha抗体, 抗-Ha抗体, 抗Ha, 抗Ha抗体, 抗-HA
MDL编号:
NACRES:
NA.43

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

technique(s)

immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates
indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells
western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts

application(s)

research pathology

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
H9658H9037B9183
Anti-HA antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

H6908

抗-HA 兔抗

Monoclonal Anti-HA antibody produced in mouse clone HA-7, ascites fluid

Sigma-Aldrich

H9658

单克隆抗-HA 小鼠抗

Anti-HA-Biotin antibody, Mouse monoclonal clone HA-7, purified from hybridoma cell culture

Sigma-Aldrich

B9183

单克隆抗-HA

conjugate

unconjugated

conjugate

unconjugated

conjugate

TRITC conjugate

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

polyclonal

clone

HA-7, monoclonal

clone

HA-7, monoclonal

clone

HA-7, monoclonal

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein

technique(s)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

technique(s)

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

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一般描述

血凝素(HA)基因定位于乙型流感病毒基因组的第4区段。HA是存在于流感病毒表面的一种主要抗原。HA是一个同源三聚体,其中每个单体均为单一的多肽。该单体会被宿主蛋白酶切割成两个亚基HA1和HA2。
流感血凝素蛋白是一种源自人流感病毒主要刺突膜糖蛋白的九肽。该菌株特异性的糖蛋白是一种84 kDa单体的同源三聚体。每种单体含有两个二硫键连接的亚基:HA1和HA2。
抗-HA是在兔中使用一种对应于偶联至KLH的人流感血凝素(HA)的氨基酸残基的一段合成肽而产生的。采用固定的免疫肽上亲和纯化抗体。

抗-HA抗体对于HA标签融合蛋白的N-端或C-端具有特异性。产品在特定的哺乳动物细胞中可能会检测到交叉反应的条带。该免疫HA肽(货号I12149)可特异性地抑制HA标记蛋白条带的染色。

特异性

抗-HA抗体对于HA标签融合蛋白的N-端或C-端具有特异性。
该抗体可特异性的通过免疫印迹和免疫沉淀于HA标签融合蛋白反应。

免疫原

与人流感病毒血凝素(HA)的氨基酸残基对应的合成肽被称为HA标签,偶联至KLH。

应用

抗-HA抗体适用于免疫沉淀和蛋白质印迹。
它还适用于间接免疫荧光(10-20μg/mL,使用HA标签融合蛋白转染的细胞)、免疫沉淀((2.5-4μg/次检测,使用来自细胞裂解物的HA标签融合蛋白)以及蛋白质印迹(0.5-0.8μg/mL,使用HA标签融合蛋白转染的细胞提取物)。

生化/生理作用

血凝素(HA)是一种流感病毒糖蛋白,可在病毒进入和感染期间调节膜融合和受体结合功能。该病毒可通过内吞作用和HA抗原介导的连续膜融合提高宿主细胞的进入。Ha在病毒发病机制和宿主对病毒感染的反应中起关键作用。
HA基因通常用作重组表达载体系统中靶蛋白的一种亲和标签。由这种系统所产生的HA标记蛋白质可产生稳定的不干扰靶蛋白质的功能和分布的融合产物。抗-HA抗体在特定的哺乳动物细胞中可能会检测到交叉反应的条带。该免疫HA肽(货号I12149)可特异性地抑制HA标记蛋白条带的染色。编码HA肽的核酸序列已被插入到各种表达质粒中并与克隆位点相邻,因此能够对HA标记的融合蛋白进行克隆和表达。这样的融合蛋白可在多种生物的细胞中表达:细菌、酵母、昆虫和哺乳动物。在融合蛋白中,HA序列可作为特异性抗体的识别靶点,从而能够实现对HA标记蛋白及相关结合蛋白进行检测、亚细胞定位、表征、定量、功能分析和亲和纯化。在细胞蛋白的不同区域中插入HA表位并随后对完整和通透化处理的细胞中表位的免疫反应性进行检查,可用于研究标记蛋白的细胞表达水平、拓扑结构和功能活性。抗体介导的HA标签检测消除了对新鉴定的、低丰度、不稳定、难以纯化、具有交叉反应性或免疫原性差的蛋白进行耗时的特异性抗体生成的需要。

外形

含有1%牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水(pH7.4)中的溶液

储存及稳定性

如需持续使用,可储存在 2-8 °C最长达一个月。
如果更长时间储存,按工作体积分装后冻存。不建议反复冻融。如果在更长时间的储存后出现了轻微的浑浊,在使用前可通过离心而使溶液澄清。稀释的工作溶液在12小时后应当被丢弃。

免责声明

除非在我们的产品目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用并不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体体内治疗用途、任何类型的食用或应用于人或动物。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the concentration of Product No. H6908, anti HA?

    The antibody concentration for Product No. H6908 is reported as a lot-specific value on the certificates of analysis. Please refer to the Certificate of Analysis for the lot received for the specific antibody concentration. Values for recent lots have ranged from 0.5 - 0.7 mg/mL.

  5. Is the immunizing peptide for Product H6908, Anti-HA antibody, available?

    The immunizing peptide is Product No. I2149. It is useful for displacement of HA-tagged fusion proteins from HA antibody in immunoassays. The successful inhibition of antibody binding by HA peptide demonstrates the binding of the antibody is specific.

  6. What strain of influenza hemagglutinin does Product H6908, Anti-HA antibody, recognize?

    The sequence of the immunizing peptide is YPYDVPDYA. This sequence corresponds to amino acids 99-107 of human influenza virus X47 (classified as an H3N2 strain) referred to in Niman, H.L., et al., Proc. Natl. Acad. Sci. USA, 80, 4949-4953, 1983. However, according to the NCBI databases, the same epitope (YPYDVPDYA) corresponds to amino acids 98-106 in the vast majority of prevalent influenza viruses hence our claim in the data sheet of the antibodies. NCBI is National Center for Biotechnology Information.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Proline isomerase Pin1 represses terminal differentiation and myocyte enhancer factor 2C function in skeletal muscle cells.
Magli A, et al
The Journal of Biological Chemistry, jbc-M110 (2010)
Frontiers in neurology and neuroscience research null
Frontiers in neurology and neuroscience research null
Functional evaluation of Dent?s disease-causing mutations: implications for ClC-5 channel trafficking and internalization.
Ludwig M, et al.
Human Genetics, 117(2-3), 228-237 (2005)
H Jin et al.
Oncogene, 35(39), 5106-5118 (2016-03-15)
The expression of immediate early response 3 (IER3), a protein with a short half-life, is rapidly induced by various cellular stimuli. We recently reported that IER3 induces the apoptosis of cervical cancer cells and that its expression is downregulated in

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