产品名称
抗-组蛋白H3（N-末端） 兔抗, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~17 kDa
species reactivity
human
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
indirect immunofluorescence: 2-4 μg/mL using A431 cells.
western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HIST1H3A(8350)
Application
兔抗组蛋白H3（N-末端）抗体还可用于蛋白质印迹和天然染色质免疫沉淀检测。
Biochem/physiol Actions
组蛋白是DNA包装的结构支架。组蛋白H3在复制和DNA修复中起重要作用。与组蛋白家族的其他成员相比，组蛋白H3的翻译后修饰更多。该基因突变会引发小脑高级胶质瘤。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途，或人类或动物任何类型的消费或使用。
General description
抗组蛋白抗体H3（N端）特异性识别人类组蛋白H3 (~17kDa)。组蛋白H3/组蛋白簇1H3家族成员a (HIST1H3A)是组蛋白家族的核心成员。HIST1H3A由定位至人类染色体6p22.2上的基因所编码。该编码蛋白质由~136个氨基酸组成。
Immunogen
对应于人组蛋白H3的1-20位氨基酸的合成肽，与KLH偶联。
Other Notes
想要使用各种技术和制备方法都能获得最佳结果，我们推荐通过滴定确定最佳工作稀释度。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Preparation Note
如需连续使用，可在 2-8°C 下储存长达一个月。对于长期储存，冷冻工作等分试样。不建议反复冻融或储存在“无霜”冰柜中。如果延长储存时间后出现轻微浑浊，则在使用前离心澄清溶液。如果未在 12 小时内使用，则应丢弃工作稀释液。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
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Nakata S, et al.
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Maksakova IA, et al.
Epigenetics & Chromatin, 4 (2011)
Phosphorylation of histone H3 Ser10 establishes a hierarchy
for subsequent intramolecular modification events
for subsequent intramolecular modification events
Liokatis S, et al.
Nature Structural and Molecular Biology, 19, 819-823 (2012)
Xiao-Yu Zheng et al.
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Application of a low-input chromatin profiling method, CUT&RUN, to FACS-purified Arabidopsis endosperm nuclei generates parental-specific genome-wide H3K27me3 landscapes with high sensitivity, specificity and reproducibility. Endosperm is an essential seed tissue with a unique epigenetic landscape. During endosperm development, differential epigenetic
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