biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
orthogonal RNAseq
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:1000-1:2500
immunogen sequence
KEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQH
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HNF1B(6928)
General description
HNF1B(肝细胞核因子 1β) 同源域是一种非典型的 POU (Pit-1、Oct-1和Unc-86) 转录因子,与脊椎动物的早期发育和胚胎存活有关。 它在胰腺、肾脏、肺、卵巢、睾丸和整个胃肠道中表达。
Immunogen
肝细胞核因子 1β 重组蛋白表位标签
Application
兔抗HNF1B抗体属Prestige抗体,经人类蛋白质图谱(HPA)计划开发和验证。每种抗体都通过针对数百种正常和疾病组织的免疫组织化学进行测试。通过单击图像库链接,可以在人类蛋白质图谱(HPA)站点上查看这些图像。还采用免疫荧光和蛋白质印迹法对抗体进行检测。想要查看有关Prestige 抗体和HPA的方案和其他实用信息,请访问 sigma.com/prestige。
在人类蛋白质图集项目网站上单击图像库链接,可以查看这些图像和庞大的数据集。我们还提供Prestige Antibodies® 抗体的实验方案和其他有用信息。
成功使用该抗体的应用以及相关同行评审论文如下所示。
免疫细胞化学(1 篇)
免疫细胞化学(1 篇)
Biochem/physiol Actions
在乳头状肾细胞肿瘤(RCT)和一些传统RCC、肾嗜酸细胞瘤、嫌色细胞RCC和正常肾脏中,HNF1B( 肝细胞核因子1 β)的表达上调。另一方面,其表达在肾母细胞瘤(Wilms' tumor)中受到抑制。它是一种肝特异性表达所必需的核蛋白。它可以结合肝脏中表达的各种基因的启动子和增强子,以及白蛋白基因的近端元件(PE)。
Features and Benefits
Prestige Antibodies®是经过高度表征和广泛验证的抗体,同时还有一个优点是其每个靶标的所有可用表征数据都可以通过位于此页面顶部产品名称下方的人类蛋白质图谱门户进行访问。Prestige Antibodies®对其他蛋白质的独特性和低交叉反应性是通过严密的抗原区域选择、亲和纯化和严格的选择来实现的。针对每一个Prestige Antibody都存在有对应的Prestige抗原对照,并可在链接区域内找到。
每个Prestige Antibody都是按照以下方法进行测试的:
每个Prestige Antibody都是按照以下方法进行测试的:
- 44例正常人类组织以及20例最常见癌症类型组织的IHC组织阵列。
- 364个人类重组蛋白片段的蛋白阵列。
Physical form
磷酸盐缓冲盐溶液,pH 7.2,含 40% 甘油和 0.02% 叠氮化钠
Other Notes
相应抗原APREST84742
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| HPA002083-100UL | 04061833814376 |
| HPA002083-25UL | 04061842780808 |