Anti-DIAPH2 antibody produced in rabbit

Diaphanous-related formin 2 (DIAPH2) is part of the diaphanous subfamily of the formin homology family of proteins. It is a nuclear protein. A defect in this gene leads to premature ovarian failure. The gene DIAPH2 is mapped to human chromosome Xq21.33.

Protein diaphanous homolog 2 recombinant protein epitope signature tag (PrEST)

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The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Diaphanous-related formin 2 (DIAPH2) interacts specifically with the distal regulatory element (DRE) of the human skeletal α-actin gene between positions -1260 and -1193, the binding site being an A/T-rich sequence element. The DRF-2 also binds well to the muscle creatine kinase (MCK) muscle-specific regulatory factor, MEF-2 binding site and to the A/T-rich regulatory element of the skeletal muscle fast-twitch troponin C gene. This troponin C site competes for DRF-2 driven expression of the skeletal α-actin gene in C2 cells. The DRF-2 site alone does not activate transcription in muscle cells when linked to the SV40 promoter. DRF-2 binding element is a MEF-2 binding site that is required but insufficient for regulation of muscle-specific skeletal α-actin gene expression by the DRE. Therefore, muscle-specific regulation of the human skeletal α-actin gene requires interactions between the other elements of the composite DRE enhancer with the protein-DNA complex formed by DRF-2.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Corresponding Antigen APREST74314

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