Merck
CN
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文件

I1284

Sigma-Aldrich

异丙基 β-D-硫代半乳糖吡喃糖苷 溶液

ReadyMade IPTG solution for Blue-white screening

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别名:
IPTG 溶液
经验公式（希尔记法）:
C9H18O5S
CAS号:
367-93-1
分子量:
238.30
Beilstein:
4631
MDL编号:
MFCD00063273
PubChem化学物质编号:
24895928
NACRES:
NA.52

等级

for molecular biology

质量水平

200

描述

non-ionic

形式

liquid

分子量

238.3

运输

dry ice

储存温度

−20°C

SMILES string

CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

相关类别

一般描述

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

应用

Isopropyl β-D-thiogalactopyranoside solution is:
  • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
  • used in its half-life and decay studies
  • suitable for induction of recombinant protein expression in systems controlled by the lac repressor

生化/生理作用

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.

原理

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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示例

T1503
货号
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Random sequences are an abundant source of bioactive RNAs or peptides
Neme R, et al.
Nature ecology & evolution, 1(6), 0127-0127 (2017)
Half-life measurements of chemical inducers for recombinant gene expression
Pasotti L, et al.
Journal of Biological Engineering, 8(1), 5-5 (2014)
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots
Sukhanova A, et al.
Protocol Exchange, 10 (2012)
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
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