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product name
StableCell™ IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
杂质
Endotoxin, tested
组分
NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes
运输
ambient
储存温度
2-8°C
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一般描述
该 IMDM 与原处方不同,因为其添加了 L-丙氨酰-L-谷氨酰胺二肽。众所周知,谷氨酰胺在细胞培养中是不稳定的。这种二肽为细胞培养提供了一种更稳定的谷氨酰胺形式。
应用
经 Guilbert 和 Iscove 证明,红细胞和巨噬细胞的前体细胞可在添加白蛋白、转铁蛋白、卵磷脂和硒的完全确定的无血清培养基中培养。该培养基是对 Dulbecco 改良 Eagle 培养基 (DME) 的改良,含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES 缓冲液和硝酸钾而不是硝酸铁。进一步研究证明,Iscove 培养基可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞。
法律信息
StableCell is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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Selective accumulation of aggregation-prone proteasome substrates in response to proteotoxic stress.
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Conditions causing an increase in misfolded or aberrant proteins can impair the activity of the ubiquitin/proteasome system (UPS). This observation is of particular interest, given the fact that proteotoxic stress is closely associated with a large variety of disorders. Although
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