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Merck
CN

I2911

Sigma-Aldrich

IMDM

StableCell, with stable glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

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别名:
Iscove 改良杜氏培养基, IMDM
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

product name

StableCell IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

杂质

Endotoxin, tested

组分

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

运输

ambient

储存温度

2-8°C

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一般描述

该 IMDM 与原处方不同,因为其添加了 L-丙氨酰-L-谷氨酰胺二肽。众所周知,谷氨酰胺在细胞培养中是不稳定的。这种二肽为细胞培养提供了一种更稳定的谷氨酰胺形式。

应用

经 Guilbert 和 Iscove 证明,红细胞和巨噬细胞的前体细胞可在添加白蛋白、转铁蛋白、卵磷脂和硒的完全确定的无血清培养基中培养。该培养基是对 Dulbecco 改良 Eagle 培养基 (DME) 的改良,含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES 缓冲液和硝酸钾而不是硝酸铁。进一步研究证明,Iscove 培养基可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞。

法律信息

StableCell is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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