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产品名称
Iscove 改良杜氏培养基, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
phenol red: yes
L-glutamine: no
glucose: yes
运输
ambient
储存温度
2-8°C
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应用
Guilbert和Iscove证明红细胞和巨噬细胞的前体细胞可以在完全确定的无血清培养基中进行培养，该培养基需补充白蛋白、转铁蛋白、卵磷脂和硒。该培养基是Dulbecco′s改良Eagle′s培养基（DME）的改良版本，其含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES缓冲液、以及硝酸钾（代替硝酸铁）。进一步的研究表明，Iscove′s培养基可以支持小鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和各种杂交细胞的成长。
重悬
以0.584 g/L补充 L-谷氨酰胺
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Sophie G Kellaway et al.
Haematologica, 106(6), 1569-1580 (2020-04-18)
Hematological malignancies are characterised by a block in differentiation, which in many cases is caused by recurrent mutations affecting the activity of hematopoietic transcription factors. RUNX1-EVI1 is a fusion protein formed by the t(3;21) translocation linking two transcription factors required
N Vriezen et al.
Biotechnology and bioengineering, 54(3), 272-286 (1997-05-05)
Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology
N N Iscove et al.
The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
Dillon M Voss et al.
Cancer research, 80(21), 4681-4692 (2020-09-16)
Muscleblind-like proteins (MBNL) belong to a family of tissue-specific regulators of RNA metabolism that control premessenger RNA splicing. Inactivation of MBNL causes an adult-to-fetal alternative splicing transition, resulting in the development of myotonic dystrophy. We have previously shown that the
Guangfeng Geng et al.
eLife, 10 (2021-05-05)
Erythropoietin (EPO) drives erythropoiesis and is secreted mainly by the kidney upon hypoxic or anemic stress. The paucity of EPO production in renal EPO-producing cells (REPs) causes renal anemia, one of the most common complications of chronic nephropathies. Although mitochondrial
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