产品名称
Iscove 改良杜氏培养基, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
表单
powder
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
组分
phenol red: yes
glucose: yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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一般描述
Iscove改良杜氏培养基是杜氏改良Eagle培养基(DME)的改良版本，含有硒、额外的氨基酸和维生素、丙酮酸钠、4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液、以及硝酸钾（代替硝酸铁）。据报道，Iscove培养基可支持鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和多种杂交细胞的生长。
应用
Iscove改良杜氏培养基已用于KG1a细胞（人白血病细胞株）和中国仓鼠卵细胞(CHO)DG44细胞株的培养。
据报道，其可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞的生长。
制备说明
补充 3.024 g/L 碳酸氢钠。
配制成每升培养基中含 17.7 克粉末。
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
此项目有
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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