产品名称
Iscove 改良杜氏培养基, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove改良杜氏培养基是杜氏改良Eagle培养基(DME)的改良版本,含有硒、额外的氨基酸和维生素、丙酮酸钠、4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液、以及硝酸钾(代替硝酸铁)。据报道,Iscove培养基可支持鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和多种杂交细胞的生长。
Application
Iscove改良杜氏培养基已用于KG1a细胞(人白血病细胞株)和中国仓鼠卵细胞(CHO)DG44细胞株的培养。
据报道,其可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞的生长。
Preparation Note
补充 3.024 g/L 碳酸氢钠。
配制成每升培养基中含 17.7 克粉末。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Ryan S Pawell et al.
Biomicrofluidics, 7(5), 56501-56501 (2014-01-10)
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a
Yogesh Kumar Verma et al.
International journal of radiation biology, 96(5), 671-688 (2020-01-28)
Purpose: Lithium chloride (LiCl) is clinically used for manic disorders. Its role has been shown in improving cell survival by decreasing Bax and p53 expression and increasing Bcl-2 concentration in the cell. This potential of LiCl is responsible for reducing
Elizabeth A Henderson et al.
Virology journal, 17(1), 43-43 (2020-04-03)
Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| I7633-10X1L | 04061833858332 |
| I7633-50K | 04061834180449 |
| I7633-10L | 04061833858325 |
| I7633-50L | 04061833858349 |