I9271
5-（碘乙酰胺基）荧光素
≥90% purity (HPLC), powder
别名:
5-IAF
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关于此项目
经验公式（希尔记法）:
C22H14INO6
化学文摘社编号:
分子量:
515.25
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Beilstein/REAXYS Number:
6543244
产品名称
5-（碘乙酰胺基）荧光素, ≥90% (HPLC)
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1
InChI
1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)
InChI key
UATCLPJEZJKNHE-UHFFFAOYSA-N
assay
≥90% (HPLC)
form
powder
technique(s)
titration: suitable
color
yellow to orange
solubility
DMSO: 5 mg/mL
fluorescence
λex 492 nm; λem 515 nm (Mercaptoethanol adduct)
λex 493 nm; λem 522 nm (pH 9.2)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
Quality Level
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Application
5-(碘乙酰氨基)荧光素用于合成荧光标记的细胞器（细胞核和核基质）、蛋白质和肽，包括酶。
General description
5-(碘乙酰氨基)荧光素是一种硫醇反应性荧光素衍生物。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The Journal of Biological Chemistry, 264, 726-726 (1989)
Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.
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Eukaryotic Cell, 9, 31-31 (2010)
Meng M Rowland et al.
Nucleic acids research, 42(14), 9295-9303 (2014-07-14)
The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed exemplar of DNA sliding is human 8-oxoguanine ((o)G) DNA glycosylase 1 (hOGG1), which
D Chhabra et al.
Biophysical journal, 89(3), 1902-1908 (2005-07-05)
Actin is the principal component of microfilaments. Its assembly/disassembly is essential for cell motility, cytokinesis, and a range of other functions. Recent evidence suggests that actin is present in the nucleus where it may be involved in the regulation of
Gábor Glatz et al.
The Journal of biological chemistry, 288(12), 8596-8609 (2013-02-06)
Mitogen-activated protein kinase (MAPK) activation depends on a linear binding motif found in all MAPK kinases (MKK). In addition, the PB1 (Phox and Bem1) domain of MKK5 is required for extracellular signal regulated kinase 5 (ERK5) activation. We present the
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