产品名称
抗-Kindlin-2 兔抗, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~72 kDa
species reactivity
mouse, rat, canine, human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2-5 μg/mL using canine MDCK cells
western blot: 1-2 μg/mL using whole extracts of human HeLa, mouse P19 or rat AT3B-1 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FERMT2(10979)
mouse ... Fermt2(218952)
rat ... Fermt2(289992)
Application
抗-kindlin-2兔抗可用于western blotting。
Biochem/physiol Actions
Kindlin-2 可作为粘附斑蛋白( focal adhesion protein ),通过促进基因组不稳定性控制乳腺癌进展。作为整联蛋白相互作用蛋白,Kindlin-2在整联蛋白的激活和整联蛋白介导的细胞-细胞外基质(ECM)相互作用中起关键作用。Kindlin-2的表达与肝细胞癌(HCC)和胰管腺癌(PDAC)患者的不良预后有关。因此,kindlin-2被认为是HCC和PDAC患者的潜在生物标志物。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
抗-kindlin-2抗体可识别人、小鼠、大鼠和狗中的kindlin-2。Kindlin-2,也称为费米铁蛋白家族成员2(FERMT2)或含pleckstrin同源结构域家族C成员1(PLEKHC1),由对应于人类染色体14q22.1的基因编码。Kindlin-2是kindlin蛋白家族的一员,具有普遍存在的表达模式。
Other Notes
为了在不同的技术和制备中获得最佳结果,我们建议通过效价测试确定最佳的工作浓度。
Physical form
溶于含有15 mM叠氮化钠的0.01 M磷酸盐缓冲液(pH 7.4)。
Preparation Note
如需连续使用,可在2-8°C下保存一个月。对于长期储存,可将工作浓度的分装品冷冻。不建议反复冻融。如果长期储存时出现轻微混浊,使用前通过离心澄清溶液。如果在12小时内未使用,则应丢弃工作稀释样品。
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Xiaowei Xue et al.
Cancer medicine, 9(14), 4971-4979 (2020-05-22)
Breast cancer was the most frequent and the second most deadly cancer in women in 2018 in China; thus, early diagnosis of breast cancer is important. Studies have reported that tissue stiffness promotes cancer progression through increased collagen or fibrosis.
Bing Li et al.
Science China. Life sciences, 62(2), 225-234 (2018-11-22)
Kindlin-2, an integrin-interacting protein, regulates breast cancer progression. However, currently, no animal model to study the role of Kindlin-2 in the carcinogenesis of mammary gland is available. We established a Kindlin-2 transgenic mouse model using a mammary gland-specific promoter, mammary
Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling
Wu X, et al.
International Journal of Molecular Medicine, 37, 436-444 (2016)
Ting Zhao et al.
Cancer letters, 330(2), 208-216 (2012-12-06)
Kindlin-2, as a focal adhesion protein, has been found to regulate tumor progression. However, the mechanism underlying Kindlin-2 regulation of tumor progression is largely unknown. Here, we report that Kindlin-2 regulates breast cancer cell proliferation, apoptosis and chromosomal abnormalities in
Prawej Mahawithitwong et al.
Pancreas, 42(4), 663-669 (2013-03-20)
Kindlin-2 is a novel focal adhesion protein reported to be expressed in breast, lung, and gastric cancers. This study aimed to investigate the significance of kindlin-2 expression in pancreatic ductal adenocarcinomas (PDACs). We performed immunohistochemical analysis on kindlin-2 on PDAC
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