L2018
脂多糖 来源于大肠杆菌 K-235
purified by gel-filtration chromatography
别名:
LPS
生物来源
Escherichia coli (K-235)
质量水平
表单
lyophilized powder
纯化方式
gel-filtration chromatography
杂质
<1% Protein
颜色
white to yellow cast
溶解性
water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
运输
ambient
储存温度
2-8°C
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一般描述
本品从大肠杆菌血清型K235中提取,经凝胶过滤纯化。来源菌株为 ATCC 13027。
生化/生理作用
脂多糖(LPS)位于膜的外层,并且在非包封的菌株中暴露在细胞表面上。它们有助于外膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的作用。
制备说明
产物可溶于水(5mg/ml)或细胞培养基(1mg/ml),产生浑浊的淡黄色溶液。在经过涡旋,并升温至 70-80oC 后,含水盐水变得更浓缩,但仍然浑浊(20mg/ml)。脂多糖分子在每种溶剂中均形成胶束。在水和磷酸盐缓冲盐水中观察到浑浊的溶液。使用有机溶剂,溶液也是浑浊的。甲醇产生具有漂浮物的浑浊悬浮液,而水则产生均匀浑浊的溶液。
警示用语:
Danger
危险声明
危险分类
Acute Tox. 2 Oral
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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