biological source
Escherichia coli (K-235)
Quality Segment
form
lyophilized powder
purified by
gel-filtration chromatography
impurities
<1% Protein
color
white to yellow cast
solubility
water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
General description
本品从大肠杆菌血清型K235中提取,经凝胶过滤纯化。来源菌株为 ATCC 13027。
Biochem/physiol Actions
脂多糖(LPS)位于膜的外层,并且在非包封的菌株中暴露在细胞表面上。它们有助于外膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的作用。
Preparation Note
产物可溶于水(5mg/ml)或细胞培养基(1mg/ml),产生浑浊的淡黄色溶液。在经过涡旋,并升温至 70-80oC 后,含水盐水变得更浓缩,但仍然浑浊(20mg/ml)。脂多糖分子在每种溶剂中均形成胶束。在水和磷酸盐缓冲盐水中观察到浑浊的溶液。使用有机溶剂,溶液也是浑浊的。甲醇产生具有漂浮物的浑浊悬浮液,而水则产生均匀浑浊的溶液。
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
