biological source
bacterial (Klebsiella pneumoniae)
form
lyophilized powder
purified by
phenol extraction
impurities
<3% Protein (Lowry)
color
white to faint yellow
solubility
water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
Quality Level
General description
该产品是从肺炎克雷伯菌通过苯酚提取而来。源菌株是 ATCC 15380。
Biochem/physiol Actions
脂多糖(LPS)位于膜的外层,并且在非包封菌株中暴露在细胞表面上。它们有助于保持外膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的作用。
Preparation Note
本产品可溶于水(5mg/ml)或细胞培养基(1mg/ml),形成浑浊的淡黄色溶液。经过涡旋并升温至 70-80 oC 后,在盐水中可获得浓度更高、但仍然混浊的溶液(20mg/ml)。脂多糖分子在每种溶剂中都会形成胶束。在水和磷酸盐缓冲液中形成浑浊的溶液。在有机溶剂中也不会更清澈。在甲醛中形成具有漂浮物的浑浊悬浮液,而在水中则产生均匀浑浊的溶液。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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商品
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
相关内容
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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