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安全信息

L5542

Sigma-Aldrich

LB Agar Plates

pre-poured LB agar plates for E. coli

别名:

pre-poured LB agar plates

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About This Item

UNSPSC代码:
41121800
NACRES:
NA.74

等级

for molecular biology

质量水平

无菌性

sterile; autoclaved

形式

plates (10 cm)
plates (10cm, 20-25ml each)

容量

20-25 mL

储存温度

2-8°C

适用性

nonselective for Escherichia coli
nonselective for coliforms

一般描述

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

应用

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
  • to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus
  • to culture numerous bacteria and is used in strain storage method
  • to analyse the egestion of midgut microbes during sand fly bites

特点和优势

Lennox LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation

组分

15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference among the many LB - Lennox products?

    LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Bacteria Detection and Differentiation Using Impedance Flow Cytometry
Clausen C, et al.
Sensors, 18(10), 3496-3496 (2018)
Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ
Kuru E, et al.
Nature Protocols, 10(1), 33-33 (2015)
Priyanka Karicherla et al.
Investigative ophthalmology & visual science, 50(1), 256-262 (2008-09-09)
Nona-D-arginine amide (D9R) was evaluated as treatment for Pseudomonas aeruginosa keratitis when administered alone and with ciprofloxacin. Mouse corneas were infected with P. aeruginosa. Immediately after infection and hourly for 5 hours, eyes received 5 microL of either Dulbecco phosphate-buffered
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem

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