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Merck
CN

M3148

2-巯基乙醇

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

别名:

β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇

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关于此项目

线性分子式:
HSCH2CH2OH
化学文摘社编号:
60-24-2
分子量:
78.13
UNSPSC Code:
12161504
NACRES:
NA.31
PubChem Substance ID:
24896800
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890

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产品名称

2-巯基乙醇, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

SMILES string

OCCS

grade

Molecular Biology

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioReagent

assay

99% (GC/titration)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: soluble 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

2-8°C

Quality Level

200

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相关类别

Application

BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键，通常用于 SDS-PAGE 的样品缓冲液，浓度为 5%。切割分子间（亚基之间）二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。切割分子内（亚基内）二硫键可使亚基完全变性，从而使每个肽根据其链长迁移，而不受二级结构的影响。
还原剂
β-巯基乙醇或2-巯基乙醇已用于：
  • 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞（鸡B细胞系）的培养
  • 裂解NuLi-1（Normal Lung, University of Iowa-1）细胞
  • 小鼠胚胎干细胞的胰蛋白酶消化

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

154.4 °F - closed cup

flash_point_c

68 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000508524
SHBT1430
BCCN4539
BCCM6691
0000385092

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实验方案

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

MTT Assay Protocol for Cell Viability and Proliferation

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四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

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