推荐产品
product name
MegaCell™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
无菌性
sterile-filtered
质量水平
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes
运输
ambient
储存温度
2-8°C
一般描述
MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.
应用
MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
- as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
- as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
- to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies
重悬
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
法律信息
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Shintaro Iwashita et al.
Bioscience reports, 35(4), doi:10-doi:10 (2015-07-17)
The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa) at the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila Yeti homologues play crucial roles in chromatin remodelling
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