M6818
Anti-MeCP2 antibody, Mouse monoclonal
clone Mec-168, purified from hybridoma cell culture
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关于此项目
Conjugate:
unconjugated
Clone:
Mec-168, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
26
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Mec-168, monoclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)
General description
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).
Immunogen
synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- immunostaining
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)
Biochem/physiol Actions
Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
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闪点 (F)
Not applicable
闪点 (°C)
Not applicable
PPE（个人防护设备）
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)