Merck
CN

M7408

Millipore

麦康凯琼脂

NutriSelect® Basic, suitable for microbiology

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别名:
肠道细菌选择培养基,MacConkey′s培养基
EC 号:
MDL编号:
eCl@ss:
42040102
NACRES:
NA.74

无菌性

non-sterile

质量水平

形式

powder

包装

bottle of 250 g
bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

颜色

light yellow

application(s)

agriculture
environmental
food and beverages
pharmaceutical
water monitoring

microbiology

适用性

Citrobacter spp.
Escherichia coli
Gardnerella spp.
coliforms

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form

powder

form

powder

form

powder

form

powder

packaging

bottle of 250 g, bottle of 500 g

packaging

-

packaging

-

packaging

-

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Basic

application(s)

agriculture
environmental
food and beverages
pharmaceutical
water monitoring, microbiology

application(s)

agriculture
environmental
food and beverages, microbiology

application(s)

agriculture
environmental
food and beverages
life science and biopharma, microbiology

application(s)

agriculture
environmental
food and beverages, microbiology

suitability

Citrobacter spp., Escherichia coli, Gardnerella spp., coliforms

suitability

selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae, selective and differential for enterovirulent E. coli (EEC)

suitability

Arthrobacter spp., nonselective for Aspergillus spp., nonselective for Bacillus spp., nonselective for Candida spp., nonselective for Penicillium spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

suitability

nonselective for Lactococcus spp., nonselective for Streptococcus spp.

一般描述

MacConkey琼脂用于乳糖发酵和非乳糖发酵肠道细菌的分离、鉴定和计数。此培养基受牛奶和乳制品及药物制剂标准检验方法认可。培养基中的蛋白胨提供含氮和含碳化合物、长链氨基酸、维生素和其他必需的生长因子。乳糖是一种可发酵糖,当培养基的 pH 值低于 6.8 时,会导致酸形成和指示剂颜色变化。氯化钠可维持渗透压平衡。该培养基的选择性作用源自结晶紫和胆盐,它们对大多数革兰氏阳性菌具有抑制作用。革兰氏阴性菌通常在培养基上生长良好,并可通过乳糖发酵能力进行区分。乳糖发酵菌株形成红色或粉红色菌落,并被酸沉淀胆汁区域包围。非乳糖发酵菌株(例如志贺氏菌和沙门氏菌)形成无色透明菌落,通常不会改变培养基的外观。

应用

MacConkey 琼脂是一种差异化铺板培养基,推荐用于检测和分离粪便、尿液、水和其他材料中的大肠菌群和肠道病原体。

生化/生理作用

麦康凯琼脂培养基被视为一种选择性和鉴别性培养基。它有助于区分乳糖发酵菌和非乳糖发酵菌。因此,可用于分离革兰氏阴性细菌。原代平板培养基用于区分大肠杆菌群的成员。

特点和优势

NutriSelect® Basic是粉状培养基产品
  • 通过基本的促生长试验进行质量控制
  • 符合 ISO 9001 标准的质量保证
  • 高成本效益的粉末培养基

制备说明

取50 g溶于1L蒸馏水中。煮沸使其完全溶解。在121°C高压灭菌15分钟消毒。倒入无菌培养皿。接种前,使凝胶表面干燥。

分析说明

已进行微生物学测试。

附注

我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

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