M7443
Anti-MeCP2 antibody, Mouse monoclonal
clone Men-8, purified from hybridoma cell culture
别名:
Anti-AUTSX3, Anti-MRX16, Anti-MRX79, Anti-MRXS13, Anti-MRXSL, Anti-PPMX, Anti-RS, Anti-RTS, Anti-RTT
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关于此项目
Conjugate:
unconjugated
Clone:
Men-8, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
23
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
Men-8, monoclonal
form
buffered aqueous solution
mol wt
antigen ~75 kDa
species reactivity
mouse, human, rat, monkey
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)
General description
Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2.
Immunogen
synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2.
Application
Monoclonal Anti-MeCP2 antibody has been used:
- in western blotting
- in immunoblotting
- in flow cytometry
- in immunohistofluorescence
- in immunocytochemistry
Biochem/physiol Actions
MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.
Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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此项目有
Paul D Ross et al.
Human molecular genetics, 25(20), 4389-4404 (2017-02-09)
Rett syndrome (RTT) is a severe genetic disorder resulting from mutations in the X-linked MECP2 gene. MeCP2 protein is highly expressed in the nervous system and deficiency in the mouse central nervous system alone recapitulates many features of the disorder.
Radically truncated MeCP2 rescues Rett syndrome-like neurological defects
Tillotson R, et al.
Nature, 550(7676), 398-398 (2017)
A codon-optimized Mecp2 transgene corrects breathing deficits and improves survival in a mouse model of Rett syndrome
Matagne V, et al.
Neurobiology of Disease, 99, 1-11 (2017)
Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice
Lawson-Yuen A, et al.
Brain Research, 1180, 1-6 (2007)
Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor
Lyst M J, et al.
Nature Neuroscience, 16(7), 898-898 (2013)
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