M7683

抗-磷酸-MAP激酶激酶1/2 (pSer^217/221) 兔抗

Name: 抗-磷酸-MAP激酶激酶1/2 (pSer<SUP>217/221</SUP>) 兔抗
Brand: SIGMA

affinity isolated antibody, buffered aqueous glycerol solution

别名:

抗-磷酸-MEK 1/2 (pSer^217/221)

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD02097160

Conjugate:

unconjugated

Clone:

polyclonal

Application:

FACS, ICC, IHC (p), IP, WB

Citations:

主要文件

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 45 kDa

species reactivity

human, rat, mouse

technique(s)

flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells., immunocytochemistry: 1:1,000 using HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma, immunoprecipitation (IP): 1:50, western blot: 1:1,000 using serum-treated NIH-3T3 cells

UniProt accession no.

P28482

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer217/pSer221)

Quality Level

200

Gene Information

human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)

General description

MAP激酶激酶（MAPKK、MEK1和MEK2）是对苏氨酸/酪氨酸残基双磷酸化激活底物的酶家族成员。MEK1和MEK2的催化结构域有86%的同源性。MEK1/2先被丝裂原刺激细胞中的Raf-1激酶活化， MEK1/2随后激活ERK1/2，进而激活多个下游靶标，参与细胞增殖、凋亡和移动。除了ERK1/2，可能还有其他非催化性的MEK1/2效应子。MEK的各种亚型在中枢神经系统、胸腺、脾脏、心脏、肺和肾脏中广泛表达。活性MEK1/2是NIH3T3细胞转化和PC-12细胞分化的充分条件。
该抗体仅特异性识别Ser²¹⁷和Ser²²¹位磷酸化的MEK1/2 (45 kDa)。它与仅Ser²¹⁷为磷酸化的MEK1/2反应强烈，但与仅Ser²²¹磷酸化的MEK1/2反应较弱。与其他相关家族成员（包括SEK）没有交叉反应。

Immunogen

对应人MEK 1/2中围绕Ser^217/221的一段残基的合成磷酸肽Ser^217/221

Application

抗-磷酸MAP激酶激酶1/2(MEK1/2)抗体应用广泛。对于血清处理的NIH3T3细胞的免疫印迹分析，推荐的工作稀释度为1:1000。对于免疫沉淀，可使用1:50的工作稀释度。对于石蜡包埋的人乳腺癌切片的免疫组织化学检测，可使用1:100的抗体稀释液。对于HeLa细胞的免疫细胞化学应用（免疫荧光），可使用1:1000的工作稀释度。

Biochem/physiol Actions

特异性识别Ser²¹⁷和Ser²²¹位磷酸化的MEK1/2。不与非磷酸化MEK 1/2、SEK (MKK4)、MKK3或MKK6反应。

Physical form

10 mM HEPES、pH 7.5溶液，含有150 mM氯化钠、100 μg/ml牛血清白蛋白和50%甘油。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

低风险生物材料

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历史批次信息供参考:

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Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells.

S Cowley et al.

Cell, 77(6), 841-852 (1994-06-17)

The MAP kinase pathway is activated by a wide variety of external signals leading to cell proliferation or differentiation. However, it is not clear whether activation of this pathway is required for cellular responses or whether it is only one

Phosphorylation regulates nucleophosmin targeting to the centrosome during mitosis as detected by cross-reactive phosphorylation-specific MKK1/MKK2 antibodies.

Hyukjin Cha et al.

The Biochemical journal, 378(Pt 3), 857-865 (2003-12-13)

Phosphorylation-specific antibodies provide a powerful tool for analysing the regulation and activity of proteins in the MAP (mitogen-activated protein) kinase and other signalling pathways. Using synchronized cells, it was observed that phosphorylation-specific antibodies developed against the active form of MKK1/MKK2

MEK1 signaling mediates transformation and metastasis of EpH4 mammary epithelial cells independent of an epithelial to mesenchymal transition.

Jan Pinkas et al.

Cancer research, 62(16), 4781-4790 (2002-08-17)

Activation of the mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)-mitogen-activated protein kinase (MAPK) pathway is a frequent event in tumorigenesis, and analysis of human breast carcinomas demonstrates that 25-50% of these tumors express elevated levels of activated MAPK1/2. However, a direct

Transformation of mammalian cells by constitutively active MAP kinase kinase.

S J Mansour et al.

Science (New York, N.Y.), 265(5174), 966-970 (1994-08-12)

Mitogen-activated protein (MAP) kinase kinase (MAPKK) activates MAP kinase in a signal transduction pathway that mediates cellular responses to growth and differentiation factors. Oncogenes such as ras, src, raf, and mos have been proposed to transform cells by prolonging the

From basic research to clinical development of MEK1/2 inhibitors for cancer therapy.

Christophe Frémin et al.

Journal of hematology & oncology, 3, 8-8 (2010-02-13)

The Ras-dependent Raf/MEK/ERK1/2 mitogen-activated protein (MAP) kinase signaling pathway is a major regulator of cell proliferation and survival. Not surprisingly, hyperactivation of this pathway is frequently observed in human malignancies as a result of aberrant activation of receptor tyrosine kinases

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抗-磷酸-MAP激酶激酶1/2 (pSer217/221) 兔抗

affinity isolated antibody, buffered aqueous glycerol solution

选择尺寸

关于此项目

主要文件

属性

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

相关类别

说明

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Disclaimer

安全信息

存储类别

flash_point_f

flash_point_c

法规信息

文件

分析证书(COA)

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抗-磷酸-MAP激酶激酶1/2 (pSer^217/221) 兔抗