biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 43 kDa
species reactivity
Arabidopsis thaliana
technique(s)
western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves, western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Arabidopsis thaliana ... ATMPK3(823706)
General description
蛋白激酶AtMPK3是MAP激酶级联反应的组成部分。
Immunogen
对应于拟南芥MPK3 C末端氨基酸的合成肽。 该序列对AtMPK3具有特异性(在其他20种已知的AtMPK同工型中找不到)。 它与AtMPK6同工型的同源性有限(同一性为58%),并且与烟草直系同源物WIPK具有显着的同源性(同一性为67%,连续表位)。
Application
兔抗AtMPK3抗体已被用于免疫印迹分析。
Biochem/physiol Actions
AtMPK3是植物MAPK信号级联放大的成分,信号级联放大在FLS2受体的下游起作用并调节对细菌和真菌感染的先天反应。作为对农杆菌感染的反应,AtMPK3参与VIP1的磷酸化和之后细菌T-DNA转移到细胞核的过程。RNAi介导的AtMPK6抑制导致AtMPK3活化时间延长。抗AtMPK3抗体对AtMPK3(43 kDa)有特异性。该产品还与烟草和虞美人中的 p56反应。在免疫印迹中,AtMPK3免疫肽(拟南芥,氨基酸359-370)可特异性抑制AtMPK3的染色。
抗 AtMPK3 识别 AtMPK3。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Preparation Note
要连续使用,请在2-8°C下保存最多一个月。 为了延长存储时间,请冻结工作等分试样。不建议重复冷冻和解冻。不建议在无霜冰箱中存放。如果长时间保存会出现轻微的浑浊,请在使用前通过离心澄清溶液。如果在12小时内不使用工作稀释液,则应将其丢弃。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses
Gao F, et al.
Science China: Life Sciences, 53(11), 1315-1321 (2010)
Armin Djamei et al.
Science (New York, N.Y.), 318(5849), 453-456 (2007-10-20)
Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to
A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis
Zhang Z, et al.
Molecular plant-microbe interactions : MPMI (2017)
Nucleoporin-Regulated MAP Kinase Signaling in Immunity to a Necrotrophic Fungal Pathogen
Genenncher B, et al.
Plant Physiology, 172(2), 1293-1305 (2016)
Tsuneaki Asai et al.
Nature, 415(6875), 977-983 (2002-03-05)
There is remarkable conservation in the recognition of pathogen-associated molecular patterns (PAMPs) by innate immune responses of plants, insects and mammals. We developed an Arabidopsis thaliana leaf cell system based on the induction of early-defence gene transcription by flagellin, a
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