M8320
Anti-MLH1 (N-terminal) 兔抗
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-COCA2, Anti-FCC2, Anti-HNPCC, Anti-MGC5172
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 80-85 kDa
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton, western blot: 0.5-1 μg/mL using Jurkat cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MLH1(4292)
mouse ... Mlh1(17350)
rat ... Mlh1(81685)
General description
MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).
MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.
MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).
Immunogen
synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.
Application
- Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.
MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome
Steinke V. et al.
Deutsches Arzteblatt International, 110(3), 32-32 (2013)
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
Wang Y, et al.
Genes & Development, 14(8), 927-939 (2000)
Association between MutL homolog 1 polymorphisms and the risk of colorectal cancer: a meta-analysis.
Haiyan Chen et al.
Journal of cancer research and clinical oncology, 141(12), 2147-2158 (2015-05-20)
As one of the most essential components of mismatch repair system, MutL homolog 1 (MLH1) plays an increasingly implicated role in initiation and promotion of colorectal carcinogenesis, with germ-line mutations in different loci. However, whether a single genetic variant in
Shinichiro Fukuhara et al.
Oncotarget, 5(22), 11297-11307 (2014-12-20)
Mismatch repair (MMR) enzymes have been shown to be deficient in prostate cancer (PCa). MMR can influence the regulation of tumor development in various cancers but their role on PCa has not been investigated. The aim of the present study
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