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安全信息

MBD0021

Sigma-Aldrich

Inactivated Bacillus subtilis

Suitable for PCR, sequencing and NGS, >10^8 bacteria/ml

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质量水平

形式

powder

浓度

>10^8 bacteria/ml

技术

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

运输

dry ice

储存温度

−20°C

一般描述

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Bacillus subtilis (B. subtilis) can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.B. subtilis is a gram positive, aerobic bacterium, rod-shaped, endospore-forming, catalase-positive. It is traditionally considered as a soil microorganism.2 Bacillus subtilis is genetically well characterized and serves as a model organism for important gram-positive pathogens such as Bacillus anthracis, Staphylococcus aureus etc.3 B. subtilis possess an extreme adaptation ability to various environmental stress conditions by modifying its motility 4, uptake of exogenous DNA 5, biofilm formation and sporulation6.Recent studies have identified and characterized B. subtilis isolates from human gastrointestinal track suggesting B.subtilis as a gut commensal.7 Moreover, B. subtilis presence in the gut was found to correlate with probiotic effects such as anti-diarrhea effect, anti-microbial effect, immune-stimulatory effect, competition with pathogens, normal flora growth stimulation and prevention of intestinal inflammation.8,9. B. subtilis beneficial effect on gut inflammation may also suggest it as potential probiotic treatment for inflammatory bowel diseases (IBD), including Crohn’s and ulcerative colitis.10 In addition, it was demonstrated that a peptide produced by B. subtilis possess antiviral effect against influenza virus.11

Read here how to use our standards to ensure data integrity for your microbiome research.

应用

Inactivated bacteria are provided at >10^8 bacteria/vial as lyophilized powder. It is recommended to avoid freeze thaw cycles of this product.

重悬

It is recommended to dissolve the lyophilized inactivated bacteria in water (Sigma Cat# W4502 ) supplemented with at least 10% glycerol (Sigma Cat# G5516). Once dissolved it is recommended to aliquot immediately and store at -20°C for up to 1 month and avoid freeze thaw cycles.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

法规信息

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Turn to our Next-Gen Sequencing Oligos (NGSO) as a potential solution if you are experiencing high levels of adapter dimer formation with custom adapters sourced from other vendors.

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

相关内容

如果您因使用其他厂商的定制接头而饱受接头二聚体之苦,可转用我们的新一代测序寡核苷酸(NGSO)作为优化解决方案。

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