MediaBoost

Studying microbial species via cutting edge genomic techniques such as next generation sequencing (NGS) has become increasingly prevalent in recent years; however, genomic methods alone cannot fully replace experimental testing of microbial species. Therefore, the ability to culture microbes in a laboratory setting remains essential for comprehensive study of these communities. Some microbial species can be challenging to culture in standard culture media. Often species of interest or a specific process require specialized media compositions, to provide an optimal environment for maximal proliferation and yield. A few examples includes

• Chemically defined minimal growth media are often used to grow bacteria for proteomics, as a base for stable isotope enrichment in NMR protein structure-function studies, but growth can be limited without the addition of an effective supplement.

• Use of chemically defined minimal growth media in the production of secreted microbial proteins to ensure reproducibility and a higher concentration of the microbial-produced protein of interest.

MediaBoost provides microbial researchers a ready-to-use media supplement to amplify growth in selected gram-positive bacteria, gram-negative bacteria, and yeast species. Application data is available for some of the strains and media combinations that have been tested with MediaBoost, including:

• Bacillus subtilis spores in LB medium
• Lactobacillus rhamnosus in LB medium
• Pseudomonas aeruginosa in M9 medium
• Saccharomyces cerevisiae in YPD, BM2, and IMDM media

Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.