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Merck
CN

MBD0055

MediaBoost

non-animal sterile microbial growth supplement

别名:

growth supplement for germination, microbial culture additive, microbial culture enhancer, microbial media supplement, minimal growth media supplement

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关于此项目

NACRES:
NB.21
UNSPSC Code:
41106212
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Quality Level

sterility

sterile

form

liquid

technique(s)

microbiological culture: suitable

shipped in

wet ice

storage temp.

2-8°C

suitability

Bacillus spp., Escherichia spp., Lactobacillus spp., Lactococcus spp., Pseudomonas spp., Saccharomyces spp., bacteria, yeasts

General description

Studying microbial species via cutting edge genomic techniques such as next generation sequencing (NGS) has become increasingly prevalent in recent years; however, genomic methods alone cannot fully replace experimental testing of microbial species. Therefore, the ability to culture microbes in a laboratory setting remains essential for comprehensive study of these communities. Some microbial species can be challenging to culture in standard culture media. Often species of interest or a specific process require specialized media compositions, to provide an optimal environment for maximal proliferation and yield. A few examples includes

  • Chemically defined minimal growth media are often used to grow bacteria for proteomics, as a base for stable isotope enrichment in NMR protein structure-function studies, but growth can be limited without the addition of an effective supplement.

  • Use of chemically defined minimal growth media in the production of secreted microbial proteins to ensure reproducibility and a higher concentration of the microbial-produced protein of interest.

Application

MediaBoost provides microbial researchers a ready-to-use media supplement to amplify growth in selected gram-positive bacteria, gram-negative bacteria, and yeast species. Application data is available for some of the strains and media combinations that have been tested with MediaBoost, including:
  • Bacillus subtilis spores in LB medium
  • Lactobacillus rhamnosus in LB medium
  • Pseudomonas aeruginosa in M9 medium
  • Saccharomyces cerevisiae in YPD, BM2, and IMDM media

Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.

Features and Benefits

  • Boosts Growth of Certain Microorganisms
  • Reduces Germination Time
  • Ready-Made, Sterile Solution
  • Protein-Free



存储类别

12 - Non Combustible Liquids

wgk

WGK 3

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William H Lewis et al.
Nature reviews. Microbiology, 19(4), 225-240 (2020-10-24)
Despite the surge of microbial genome data, experimental testing is important to confirm inferences about the cell biology, ecological roles and evolution of microorganisms. As the majority of archaeal and bacterial diversity remains uncultured and poorly characterized, culturing is a
Jean-Christophe Lagier et al.
Clinical microbiology reviews, 28(1), 208-236 (2015-01-09)
A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere
Anna Gąciarz et al.
Microbial cell factories, 16(1), 108-108 (2017-06-18)
The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies



全球贸易项目编号

货号GTIN
MBD0055-10ML04065270877970