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Merck
CN

MBD0056

Sigma-Aldrich

Trace Elements Ready Made Solution

100X, microbial growth medium supplement

别名:

micronutrient

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UNSPSC代码:
41106212
NACRES:
NA.54

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质量水平

200

无菌性

0.2 μm filtered

表单

liquid

保质期

2 yr

包装

pkg of 50 mL

储存条件

protect from light

浓度

100 ×

适用性

suitable for (Media supplement)

储存温度

2-8°C

相关类别

一般描述

Minerals and vitamins are broadly used to supplement growth media in order to support growth of a variety of different bacteria. The growth media mimics the natural environment of the bacteria thus providing an optimal setting for high throughput cultures. Trace elements ready-made solution, MBD0056, is comprised of vital minerals to support critical bacterial development. The solution is based on Wolin and Wolfe’s recipe, which is especially suitable for culturing anaerobic bacteria of the human microbiome ( )

特点和优势

  • 0.2uM filtered for removal of contaminants
  • Saves time in preparation of media
  • Improved accuracy: composition and concentrations are analyzed and established by inductively coupled plasma (ICP)
  • Ready-made liquid mixture containing all ingredients.
  • Decreases exposure to chemical and toxic risks associated with the powder form of the trace elements

其他说明

50 mL Trace elements solution:
  • EDTA : 570 mg/L
  • CuSO4. 5H2O :10 mg/L
  • AlK(SO4)2 · 12H2O : 20 mg/L
  • H3BO3 :10 mg/L
  • Na2MoO4. 2H2O : 10 mg/L
  • Na2SeO3 : 1 mg/L
  • Na2WO4 . 2H2O : 10 mg/L
  • NiCl2 . 6H2O : 20 mg/L
  • MgSO4 . 7H2O : 3000 mg/L
  • MnSO4 . H2O : 500 mg/L
  • NaCl : 1000 mg/L
  • NH4Fe(SO4)2 · 12H2O :170 mg/L
  • Co(NO3)2 . 6H2O : 100 mg/L
  • CaCl2 · 2H2O :100 mg/L
  • ZnSO4 . 7H2O :100 mg/L
Add to pre-sterilized and cooled growth media. Concentration 100x.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000486101
0000486101
0000452253
0000452253
0000415095

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Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria
Ito, Tamaki, et al.
Gut Microbes, 10.1, 77-91 (2019)
Viologen dye inhibition of methane formation by Methanobacillus omelianskii
Wolin, E. A., R. S. Wolfe, and M. J. Wolin
Journal of Bacteriology, 87(5), 993-998 (1964)
Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice
Goodman, Andrew L., et al.
Proceedings of the National Academy of Sciences of the USA, 108(15), 6252-6257 (2011)
Methanogens: reevaluation of a unique biological group
Balch, W. E., et al.
Microbiological Reviews, 43(2), 260-296 (1979)

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