产品名称
Trace Elements Ready Made Solution, 100X, microbial growth medium supplement
sterility
0.2 μm filtered
form
liquid
shelf life
2 yr
packaging
pkg of 50 mL
storage condition
protect from light
concentration
100 ×
suitability
suitable for (Media supplement)
storage temp.
2-8°C
Quality Level
Features and Benefits
- 0.2uM filtered for removal of contaminants
- Saves time in preparation of media
- Improved accuracy: composition and concentrations are analyzed and established by inductively coupled plasma (ICP)
- Ready-made liquid mixture containing all ingredients.
- Decreases exposure to chemical and toxic risks associated with the powder form of the trace elements
General description
Minerals and vitamins are broadly used to supplement growth media in order to support growth of a variety of different bacteria. The growth media mimics the natural environment of the bacteria thus providing an optimal setting for high throughput cultures. Trace elements ready-made solution, MBD0056, is comprised of vital minerals to support critical bacterial development. The solution is based on Wolin and Wolfe’s recipe, which is especially suitable for culturing anaerobic bacteria of the human microbiome ( )
Other Notes
50 mL Trace elements solution:
- EDTA : 570 mg/L
- CuSO4. 5H2O :10 mg/L
- AlK(SO4)2 · 12H2O : 20 mg/L
- H3BO3 :10 mg/L
- Na2MoO4. 2H2O : 10 mg/L
- Na2SeO3 : 1 mg/L
- Na2WO4 . 2H2O : 10 mg/L
- NiCl2 . 6H2O : 20 mg/L
- MgSO4 . 7H2O : 3000 mg/L
- MnSO4 . H2O : 500 mg/L
- NaCl : 1000 mg/L
- NH4Fe(SO4)2 · 12H2O :170 mg/L
- Co(NO3)2 . 6H2O : 100 mg/L
- CaCl2 · 2H2O :100 mg/L
- ZnSO4 . 7H2O :100 mg/L
Add to pre-sterilized and cooled growth media. Concentration 100x.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice
Goodman, Andrew L., et al.
Proceedings of the National Academy of Sciences of the USA, 108(15), 6252-6257 (2011)
Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria
Ito, Tamaki, et al.
Gut Microbes, 10.1, 77-91 (2019)
Viologen dye inhibition of methane formation by Methanobacillus omelianskii
Wolin, E. A., R. S. Wolfe, and M. J. Wolin
Journal of Bacteriology, 87(5), 993-998 (1964)
Methanogens: reevaluation of a unique biological group
Balch, W. E., et al.
Microbiological Reviews, 43(2), 260-296 (1979)
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