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经验公式(希尔记法):
C22H14N6Na2O9S2
化学文摘社编号:
分子量:
616.49
EC Number:
213-903-1
UNSPSC Code:
41105322
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5374263
Colour Index Number:
20470
product line
BioReagent
Quality Segment
form
powder
composition
Dye content, ~85%
technique(s)
protein staining: suitable
impurities
≤10% water
suitability
suitable for electrophoresis
SMILES string
[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key
AOMZHDJXSYHPKS-DROYEMJCSA-L
Application
萘酚蓝黑已被用于蛋白质印迹膜的染色,可用于检测转移至膜的所有蛋白质。
Biochem/physiol Actions
萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后,建议将蛋白固定在凝胶中。然后可以将凝胶用0.1%萘酚蓝黑在7%(v/v)的乙酸中染色至少2小时,并用7%(v/v)乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20%。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1B
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
