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Merck
CN

N3393

萘酚蓝黑

BioReagent, suitable for electrophoresis

别名:

氨基黑 10B, 水牛黑色 NBR, 酸性黑1

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关于此项目

经验公式(希尔记法):
C22H14N6Na2O9S2
化学文摘社编号:
分子量:
616.49
EC Number:
213-903-1
UNSPSC Code:
41105322
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5374263
Colour Index Number:
20470
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product line

BioReagent

Quality Level

form

powder

composition

Dye content, ~85%

technique(s)

protein staining: suitable

impurities

≤10% water

suitability

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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Application

萘酚蓝黑已被用于蛋白质印迹膜的染色,可用于检测转移至膜的所有蛋白质。

Biochem/physiol Actions

萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后,建议将蛋白固定在凝胶中。然后可以将凝胶用0.1%萘酚蓝黑在7%(v/v)的乙酸中染色至少2小时,并用7%(v/v)乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20%。

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1B

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
M D Chengalroyen et al.
World journal of microbiology & biotechnology, 29(4), 625-633 (2012-11-29)
Investigation into the biodegradative capability of several actinomycetes led to the discovery of many strains possessing an ability to decolorize a variety of azo and triphenylmethane dyes. Of particular interest is an Amycolatopsis sp. isolate which displayed the ability to
Shih-Hsien Chang et al.
Journal of hazardous materials, 166(2-3), 1279-1288 (2009-01-23)
Degradation of Remazol Brilliant Blue R (anthraquinone dye) and Acid Black 1 (azo dye) by Fenton oxidation and low-cost Fe(0)/air process was compared. The doses of Fenton reagent needed for decolorization of Remazol Brilliant Blue R was much higher than
Amit Parikh et al.
Biotechnology letters, 27(5), 323-326 (2005-04-19)
Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26

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全球贸易项目编号

货号GTIN
N3393-100G04061834116011
N3393-25G04061834116028

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