Anti-NPTII antibody produced in rabbit

Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.

synthetic peptide corresponding to amino acids 133-150 located at the mid-region of E. coli NPTII, conjugated to KLH.

Anti-NPTII antibody has been used in immunoblotting.

The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.

The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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