N8664
Anti-NONO (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-NMT55, Anti-NRB54, Anti-Non-pou domain-containing octamer-binding protein, Anti-Nuclear RNA-binding protein, 54-KD, Anti-p54nrb
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
11
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells, western blot: 1-2 μg/mL using NIH-3T3 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NONO(4841)
General description
Non-POU domain containing octamer binding (NONO) is ubiquitously expressed and comprises of two tandem RNP-type RNA- recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.
Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.
Application
Anti-NONO (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation.
Biochem/physiol Actions
NONO (also termed as p54nrb) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA and DNA relaxation. NONO was suspected to be involved in pre-mRNA splicing. Later it was found that NONO and PSF mediate different functions depending on their intracellular location.
Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Prefrontal cortex shotgun proteome analysis reveals
altered calcium homeostasis and immune system
imbalance in schizophrenia
altered calcium homeostasis and immune system
imbalance in schizophrenia
Daniel Martins-de-Souza
European Journal of Anaesthesiology. Supplement (2009)
A crystallographic study of human NONO (p54nrb): overcoming pathological problems with purification, data collection and noncrystallographic symmetry
Knott GJ, et al.
Acta crystallographica. Section D, Structural biology, 761-761 (2016)
Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation
Nader A
PLoS ONE (2015)
NONO regulates the intra-S-phase checkpoint in response to UV radiation.
Alfano L
Oncogene (2016)
Paraspeckles: a novel nuclear domain.
Fox AH, et al.
Current Biology, 12(1), 13-25 (2002)
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