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Conjugate:
unconjugated
Clone:
NOS-IN, monoclonal
Application:
microarray
western blot
western blot
Species reactivity:
mouse, rat
Citations:
20
Technique(s):
microarray: suitable
western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ
western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ
Uniprot accession no.:
产品名称
单克隆抗 一氧化氮合酶,诱导型 小鼠抗, clone NOS-IN, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NOS-IN, monoclonal
mol wt
antigen 130 kDa
contains
15 mM sodium azide
species reactivity
mouse, rat
packaging
antibody small pack of 25 μL
technique(s)
microarray: suitable
western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)
Application
单克隆抗诱导型一氧化氮合酶(iNOS)可用于:
- 免疫荧光染色
- 免疫金电子显微镜
- 免疫印迹
- 免疫组化
- 免疫细胞化学
- 酶联免疫吸附测定(ELISA)
Biochem/physiol Actions
一氧化氮合酶(NOS)是一种参与合成一氧化氮(NO)的酶,而一氧化氮是在生理条件下几乎所有哺乳动物细胞都能产生的自由基。NO是由精氨酸在NOS的作用下形成的,NOS氧化精氨酸的胍基氮,并释放出NO和瓜氨酸。NO是一种信使分子,介导多种功能,包括血管舒张、神经传递以及抗微生物和抗肿瘤活性。各种类型的NOS可能具有多种多样的生物学途径。
单克隆抗一氧化氮合酶诱导型 (iNOS) 抗体与诱导型一氧化氮合酶(iNOS,也称为 macNOS 和 mNOS)发生特异性反应。它不与来源于脑的 NOS 反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
单克隆抗诱导型一氧化氮合酶(iNOS)(小鼠IgG1亚型)来源于小鼠骨髓瘤细胞和免疫BALB/c小鼠脾细胞融合而产生的NOS-IN杂交瘤。根据分子量、亚细胞位置和Ca2 +依赖性,一氧化氮合酶(NOS)分为三种类型。I型NOS (150-160 kDa) 存在于神经元中。它也被称为NOS-1、神经元NOS(nNOS)、大脑NOS(bNOS)、脑NOS、组成型NOS或Ca2 +调节型NOS(cNOS)。 II型(130 kDa),主要存在于巨噬细胞,也称为巨噬细胞NOS(mNOS)或诱导型NOS(iNOS)。III型(135 kDa)存在于内皮细胞。它被称为内皮型NOS(eNOS或ecNOS)。II型NOS不依赖Ca2 +,并在刺激后在活化的巨噬细胞和某些神经胶质细胞中表达。 iNOS还存在于其他几种细胞中,包括肝细胞、软骨细胞、内皮细胞和成纤维细胞。
Immunogen
小鼠巨噬细胞(iNOS,也称为 macNOS)一氧化氮合酶 (NOS) 1126-1144 氨基酸相应的的合成肽与 KLH 结合。免疫原序列在大鼠 iNOS 中高度保守,与人 iNOS 不同。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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