NA2100

GenElute^™ 细菌基因组DNA试剂盒

Name: GenElute<SUP>™</SUP> 细菌基因组DNA试剂盒
Brand: SIGMA

sufficient for 10 purifications

别名:

细菌基因组DNA, Gen Elute

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关于此项目

UNSPSC代码:

41105501

NACRES:

NA.55

主要文件

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技术服务

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技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

用途

sufficient for 10 purifications

质量水平

200

储存温度

15-25°C

一般描述

GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌，本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液，我们提供了一种稀释的革兰氏阳性溶解溶液。

该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

Click here to view the kit protocol

应用

纯化所得的细菌基因组DNA可用于以下游应用：

限制性内切酶酶切
PCR
Southern印迹
克隆

生化/生理作用

细菌首先在含有离液盐的溶液之中裂解，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。

预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A₂₆₀/A₂₈₀比率介于1.6-1.9之间，最大长度可达50 kb。

特点和优势

典型DNA得率：15 μg - 20 μg
提供有适用于革兰氏阳性菌和革兰氏阴性菌的实验方案
可在2小时内获得优质基因组DNA
可纯化出A₂₆₀/A₂₈₀ 比率介于1.6-1.9之间的DNA

起始材料：不超过1.5 mL培养物
预期得率：至多20 μg
洗脱体积：400 μl
所需时间：70 - 120分钟
A₂₆₀/A₂₈₀比率：1.6 - 1.9
无需苯酚、氯仿或乙醇沉淀步骤

包装

Components included:

Qty in kit:3 Part number: T5449 Component description: Collection Tubes, 2 mL
Qty in kit:1 Part number: B6803 Component description: Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0)
Qty in kit:1 Part number: W0263 Component description: Wash Solution 1
Qty in kit:1 Part number: B6553 Component description: Wash Solution Concentrate
Qty in kit:1 Part number: P2308 Component description: Proteinase K
Qty in kit:1 Part number: R6148 Component description: RNase A solution
Qty in kit:1 Part number: CP9471 Component description: GenElute Miniprep Binding Columns
Qty in kit:1 Part number: L7539 Component description: Gram-Positive Lysis Solution
Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution
Qty in kit:1 Part number: B8803 Component description: Lysis Solution C
Qty in kit:1 Part number: B6678 Component description: Lysis Solution T

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号

说明

化学品安全说明书

W0263Wash Solution 1化学品安全说明书

C2112Column Preparation Solution化学品安全说明书

P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein化学品安全说明书

R6148RNase A solution化学品安全说明书

象形图

GHS02,GHS08,GHS05,GHS07

警示用语：

Danger

危险声明

H226,H290,H302,H315,H319,H334,H335,H336

预防措施声明

P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338

危险分类

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Central nervous system, Respiratory system

储存分类代码

3 - Flammable liquids

法规信息

危险化学品

常规特殊物品

低风险生物材料

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Stable multipartite mutualistic associations require that all partners benefit. We show that a single mutational step is sufficient to turn a symbiotic bacterium from an inedible but host-beneficial secondary metabolite producer into a host food source. The bacteria's host is

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实验方案

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™细菌基因组DNA试剂盒实验方案（NA2100、NA2110、NA2120）

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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GenElute^™ 细菌基因组DNA试剂盒

sufficient for 10 purifications