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Merck
CN

O3756

Sigma-Aldrich

橙黄 G

for NA electrophoresis

别名:

1-苯基偶氮基-2-萘酚-6,8-二磺酸 二钠盐, 7-羟基-8-苯基偶氮基-1,3-萘二磺酸 二钠盐, Wool Orange 2G, 酸性橙 10

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About This Item

经验公式(希尔记法):
C16H10N2Na2O7S2
CAS号:
分子量:
452.37
颜色索引号:
16230
Beilstein:
4120705
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

形式

powder

技术

nucleic acid detection: suitable

溶解性

water: 1 mg/mL, clear, orange to red

适用性

suitable for gel electrophoresis

储存温度

room temp

SMILES字符串

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

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相关类别

一般描述

橙色 G是性质呈酸性的偶氮染料 广泛用作核酸凝胶电泳的示踪染料,显示琼脂糖凝胶中DNA前端的位置。通常在浓度最高为2.5%的琼脂糖中对50bp进行显色。

应用

Orange G是一种用于核酸电泳的跟踪染料,可用于跟踪琼脂糖凝胶中的前端DNA。也作为标准品用于SDS-毛细管电泳。通常在高至2.5%的琼脂糖中显示为50bp。
橙色 G用作DNA凝胶上样染料。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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P C Dabas et al.
Journal of capillary electrophoresis, 4(5), 253-256 (1998-09-02)
A simple and reproducible micellar electrokinetic chromatography (MEKC) method has been developed for the quantitation of suramin serum levels to be used for its therapeutic drug monitoring (TDM). A running buffer solution of 20 mM sodium borate, 75 mM sodium
Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell
Nagano T, et al.
Nature Protocols, 10(12), 1986-1986 (2015)
A Hiraoka et al.
Journal of chromatography. B, Biomedical sciences and applications, 697(1-2), 141-147 (1997-10-29)
Proteins of low molecular (M(r)) in human cerebrospinal fluid (CSF) were analyzed by capillary electrophoresis in a sodium dodecyl sulfate-containing polymer solution. Under the conditions employed, peaks of beta 2-microglobulin (beta MG) (M(r): 11,700), gamma-trace protein (12,300), myelin basic protein
Touchdown PCR for increased specificity and sensitivity in PCR amplification
Korbie DJ and Mattick JS
Nature Protocols, 3(9), 1452-1452 (2008)
Differential salt fractionation of nuclei to analyze chromatin-associated proteins from cultured mammalian cells
Herrmann C, et al.
Bio-protocol, 7(6), 1986-1986 (2017)

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