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Merck
CN

O5500

四氧化锇

Sealed ampule.

别名:

“锇酸", 氧化锇(VIII)

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关于此项目

线性分子式:
OsO4
化学文摘社编号:
分子量:
254.23
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
244-058-7
MDL number:
Form:
powder
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vapor density

8.8 (vs air)

Quality Level

vapor pressure

7 mmHg ( 20 °C)

form

powder

reaction suitability

reagent type: oxidant

bp

130 °C (lit.)

mp

39.5-41 °C (lit.)

SMILES string

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

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General description

四氧化锇(OsO4)是四面体构型的对称分子,含有第三过渡系过渡金属锇。四氧化锇可作为电镜的染料和非凝固型固定剂,主要用于组织病理学实验室中的生物组织检测。标本用四氧化锇再固定(postfix)可确保脂质保存。

Application

四氧化锇已作为固定剂用于短杆菌Brevibacterium mcbrellneri处理,以及经姜黄素处理的人癌细胞系的扫描电镜观察。还可作为固定剂用于弓形虫Toxoplasma gondii骨骼肌的超微结构分析。

Biochem/physiol Actions

四氧化锇化学性结合脂质,使其不溶;固定后仅余7%的脂质可提取。因此,用四氧化锇固定后,由磷脂组成的细胞膜会变黑且电子密度提高。它还可在石蜡处理过程中固定切片中的少量脂肪。

Other Notes

由于四氧化锇的危害性,建议在使用该化学品时使用安瓿开启器。

pictograms

Skull and crossbonesCorrosion

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

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Manuel Marx et al.
Nature protocols, 7(2), 394-407 (2012-02-04)
In this report, we describe a reliable protocol for biocytin labeling of neuronal tissue and diaminobenzidine (DAB)-based processing of brain slices. We describe how to embed tissues in different media and how to subsequently histochemically label the tissues for light
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The Journal of general virology, 92(Pt 11), 2485-2493 (2011-07-29)
Negatively stained influenza virions sometimes show irregular morphology and are often referred to as pleomorphic. However, this irregular morphology has not been visualized when ultrathin-section transmission and scanning electron microscopies are used. This study focused on the effects of ultracentrifugation
F Wyss et al.
Veterinary pathology, 52(6), 1235-1242 (2015-01-27)
Pododermatitis is frequent in captive flamingos worldwide, but little is known about the associated histopathologic lesions. Involvement of a papillomavirus or herpesvirus has been suspected. Histopathologic evaluation and viral assessment of biopsies from 19 live and 10 dead captive greater
Carsten Købler et al.
Analytical and bioanalytical chemistry, 406(16), 3863-3873 (2014-01-23)
Ultrastructural characterisation is important for understanding carbon nanotube (CNT) toxicity and how the CNTs interact with cells and tissues. The standard method for this involves using transmission electron microscopy (TEM). However, in particular, the sample preparation, using a microtome to
Stephanie J Bissel et al.
Journal of neuropathology and experimental neurology, 74(8), 767-777 (2015-06-27)
Human parechovirus 3 (HPeV3) is a picornavirus associated with neurologic disease in neonates. Human parechovirus 3 infection of preterm and term infants is associated with seizures and destructive periventricular white matter lesions. Despite unremarkable cerebrospinal fluid (CSF), HPeV3 RNA can

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