P1873
抗-围脂滴蛋白 A/B 兔抗
affinity isolated antibody, buffered aqueous solution
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Citations:
54
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~62 kDa (also band at 46 kDa)
species reactivity
mouse
technique(s)
indirect immunofluorescence: 5-10 μg/mL using differentiated mouse NIH3T3-L1 cells, western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of differentiated mouse NIH3T3-L1 cells
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PLIN(5346)
mouse ... Plin(103968)
rat ... Plin(25629)
General description
脂被蛋白是白色和褐色脂肪细胞内用于贮存中性脂质的脂滴的表面蛋白。肾上腺皮质、卵巢中的类固醇生成细胞和睾丸间质细胞存在内含胆固醇酯的较小脂滴，其表面也包被少量的脂被蛋白。
Immunogen
对应于小鼠和大鼠外周血脂蛋白 A/B 的氨基酸残基 6-17 的合成肽，C 端添加半胱氨酸，与 KLH 偶联。与人相应的序列相差 2 个氨基酸。
Application
兔多克隆抗围脂滴蛋白 A/B 抗体用于标记围脂滴蛋白 A/B，以通过免疫细胞化学和免疫组化（IHC）技术进行检测和定量。它用作检测围脂滴蛋白 A/B 在脂肪细胞中三酰基甘油储存调节中的存在和作用的探针。
兔抗脂被蛋白A/B抗体可用于免疫印迹和免疫荧光染色。
Biochem/physiol Actions
兔多克隆抗围脂滴蛋白 A/B 抗体通过免疫印迹和间接免疫荧光识别小鼠 Perilipin A/B（46 kDa 和约 62 kDa）。通过免疫印迹检测围脂滴蛋白 A 和 B 条带可被免疫肽特异性抑制。
脂滴包被蛋白（perilipin，脂被蛋白）可作为分子开关，通过保护甘油三酯防止其水解而抑制基础的脂肪分解，参与调控脂肪细胞的甘油三酯贮存。脂滴包被蛋白还通过激素敏感脂肪酶 (HSL) 和非HSL来增强cAMP依赖蛋白激酶 (PKA) 激活的脂肪分解。脂被蛋白敲除小鼠的脂肪组织减少，并且能抵制饮食导致的肥胖。它们的脂质贮存脂滴包被未被PKA磷酸化的脂肪分化相关蛋白（ADRP，adipophilin）。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Masakazu Yamamoto et al.
Stem cell reports, 10(3), 956-969 (2018-02-27)
MyoD and Myf5 are fundamental regulators of skeletal muscle lineage determination in the embryo, and their expression is induced in satellite cells following muscle injury. MyoD and Myf5 are also expressed by satellite cell precursors developmentally, although the relative contribution
The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis
Sztalryd C and Brasaemle DL
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(10), 1221-1232 (2017)
Aurelia Defour et al.
Human molecular genetics, 26(11), 1979-1991 (2017-03-24)
Repair of skeletal muscle after sarcolemmal damage involves dysferlin and dysferlin-interacting proteins such as annexins. Mice and patient lacking dysferlin exhibit chronic muscle inflammation and adipogenic replacement of the myofibers. Here, we show that similar to dysferlin, lack of annexin
Christian Elabd et al.
Nature communications, 5, 4082-4082 (2014-06-11)
The regenerative capacity of skeletal muscle declines with age. Previous studies suggest that this process can be reversed by exposure to young circulation; however, systemic age-specific factors responsible for this phenomenon are largely unknown. Here we report that oxytocin--a hormone
Lijun Wang et al.
PLoS biology, 16(11), e2006522-e2006522 (2018-11-14)
During the aging process, bone marrow mesenchymal stem cells (BMSCs) exhibit declined osteogenesis accompanied by excess adipogenesis, which will lead to osteoporosis. Here, we report that the H3 lysine 36 trimethylation (H3K36me3), catalyzed by histone methyltransferase SET-domain-containing 2 (SETD2), regulates
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