biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
C-11, monoclonal
form
buffered aqueous solution
technique(s)
immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Cytokeratin, pan (mouse IgG1 isotype) is derived from the C-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cytokeratin is a member of the intermediate filaments family.
Application
Monoclonal Anti-Cytokeratin pan antibody has been used in immunoblotting, dot blot, immunohistochemistry, immunocytochemistry, immunofluorescence, immunostaining, detecting micro-metastases in lymph nodes, bone marrow, and other tissues and for determining the origin of poorly differentiated tumors.
Biochem/physiol Actions
Cytokeratin plays a major role in observing carcinomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)