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NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SPA-27, monoclonal
Application:
immunocytochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Technique(s):
immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable
indirect ELISA: 1:20,000
western blot: suitable
Citations:
19
产品名称
单克隆抗-蛋白 A 小鼠抗, clone SPA-27, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SPA-27, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
小鼠抗蛋白A单克隆抗体可用于酶联免疫吸附测定(ELISA)。它还可用于蛋白质印迹和斑点印迹分析。
Biochem/physiol Actions
对蛋白A具有特异性,与蛋白G无交叉反应性。与游离蛋白A或IgG结合蛋白A反应,不干扰蛋白A的Fc结合活性。
由于蛋白A对许多哺乳动物免疫球蛋白的Fc区具有亲和力,因此被认为是生物化学和免疫学中的通用试剂。它可用于不同的应用,例如通过亲和色谱法纯化免疫球蛋白,细胞表面研究,放射免疫测定(RIA),酶免疫测定(EIA),免疫沉淀和许多其他程序。它可以以其天然形式或与各种标记物偶联的形式用于这些方法。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
蛋白A是从金黄色葡萄球菌Cowan I菌株的细胞壁分离的42 kDa单链多肽。
Immunogen
来自金黄色葡萄球菌Cowan I菌株的蛋白A。
Physical form
小鼠中产生的单克隆抗蛋白A抗体以含15 mM叠氮化钠的腹水形式提供。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Dane Vassiliadis et al.
BMC genomics, 20(1), 251-251 (2019-03-30)
Optimal glucose metabolism is central to the growth and development of cells. In microbial eukaryotes, carbon catabolite repression (CCR) mediates the preferential utilization of glucose, primarily by repressing alternate carbon source utilization. In fission yeast, CCR is mediated by transcriptional
Jasdeep S Nanra et al.
Human vaccines & immunotherapeutics, 9(3), 480-487 (2012-12-20)
Staphylococcus aureus can cause severe life threatening invasive diseases. The principal immune effector mechanism by which humans are protected from Gram positive bacteria such as S. aureus is antigen specific antibody- and complement-dependent opsonophagocytosis. This process can be measured in
Matthew B Frankel et al.
Molecular microbiology, 78(1), 238-252 (2010-10-07)
The human pathogen Staphylococcus aureus requires cell wall anchored surface proteins to cause disease. During cell division, surface proteins with YSIRK signal peptides are secreted into the cross-wall, a layer of newly synthesized peptidoglycan between separating daughter cells. The molecular
Stephanie Marroquin et al.
Infection and immunity, 87(5) (2019-03-06)
Numerous factors have, to date, been identified as playing a role in the regulation of Agr activity in Staphylococcus aureus, including transcription factors, antisense RNAs, and host elements. Herein we investigated the product of SAUSA300_1984 (termed MroQ), a transmembrane Abi-domain/M79
A simple and robust method for the complete dissociation of HIV-1 p24 and other antigens from immune complexes in serum and plasma samples
Steindl F, et al.
Journal of Immunological Methods, 217(1-2), 143-151 (1998)
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