P3644
L-α-磷脂酰胆碱
from soybean, Type IV-S, ≥30% (enzymatic)
别名:
1,2-二酰基-sn-甘油-3-胆碱磷酸, 3-sn-磷脂酰胆碱, L-α-卵磷脂, Azolectin, PC
SMILES string
[P](=O)([O-])(OC[C@H](OC(=O)CCCCCCC\C=C/C\C=C/CCCCC)COC(=O)CCCCCCCCCCCCCCC)OCC[N+](C)(C)C
InChI key
JLPULHDHAOZNQI-ZTIMHPMXSA-N
InChI
1S/C42H80NO8P/c1-6-8-10-12-14-16-18-20-21-23-25-27-29-31-33-35-42(45)51-40(39-50-52(46,47)49-37-36-43(3,4)5)38-48-41(44)34-32-30-28-26-24-22-19-17-15-13-11-9-7-2/h14,16,20-21,40H,6-13,15,17-19,22-39H2,1-5H3/b16-14-,21-20-/t40-/m1/s1
biological source
soybean
type
Type IV-S
form
solid
concentration
≥30% (enzymatic)
solubility
chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange
functional group
phospholipid
lipid type
phosphoglycerides
shipped in
ambient
storage temp.
−20°C
Quality Level
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Application
L-α-磷脂酰胆碱适用于：
- 作为人类红细胞大量扩增培养（HEMAdef）培养基的组份，用于人成红细胞的临床扩增
- 氯离子流出试验，检测可溶性 CLIC1 引起的脂质囊泡的氯离子渗透性
- 测定溶解的蜡合酶活性的试验
- 作为缓冲液 A 的组分，用于重悬腺苷酸环化酶的胆酸盐溶解催化单元
- 作为冷冻反刍动物精子的冷冻保护剂
- 作为评估磷脂酶活性的底物
- 制备脂质体，评估其 ARF（ADP 核糖基化因子）和 AP-1（衔接蛋白）的募集效率
- 制备可溶性腺苷酸环化酶的分离催化单元
Biochem/physiol Actions
磷脂酰胆碱是真核细胞中的主要膜磷脂，形成这些膜的结构组分。它还用作几种脂质信使的储库和几种生物活性脂质的来源，例如溶血磷脂酰胆碱、磷脂酸、二酰基甘油、溶血磷脂酰胆碱、血小板活化因子和花生四烯酸。
脑磷脂脑中的主要结构磷脂，包含脂肪总量的约15%；主要局限于灰质。
Preparation Note
纯化自产品 P 5638
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
K D Lardizabal et al.
Plant physiology, 122(3), 645-655 (2000-03-11)
Wax synthase (WS, fatty acyl-coenzyme A [coA]: fatty alcohol acyltransferase) catalyzes the final step in the synthesis of linear esters (waxes) that accumulate in seeds of jojoba (Simmondsia chinensis). We have characterized and partially purified this enzyme from developing jojoba
Hossein Salmani et al.
Cryobiology, 68(2), 276-280 (2014-02-20)
Soybean lecithin is a suitable plant-based cryoprotectant for freezing ruminant sperm. Optimum level of lecithin was not clear for goat semen cryopreservation. The objective of this study was to investigate the effects of different levels of soybean lecithin in semen
R S Salter et al.
The Journal of biological chemistry, 256(19), 9830-9833 (1981-10-10)
The catalytic and guanine nucleotide regulatory (G/F) units of solubilized bovine brain adenylate cyclase were separated by gel filtration as described by Strittmatter, S., and Neer, E. J. ((1980) Proc. Natl. Acad. Sci. U. S. A. 77, 6344-6348). The isolated
Anna Kloda et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(5), 1540-1545 (2007-01-24)
In this study, the heteromeric N-methyl-D-aspartate (NMDA) receptor channels composed of NR1a and NR2A subunits were expressed, purified, reconstituted into liposomes, and characterized by using the patch clamp technique. The protein exhibited the expected electrophysiological profile of activation by glutamate
Annie Frelet-Barrand et al.
PloS one, 5(1), e8746-e8746 (2010-01-26)
Despite their functional and biotechnological importance, the study of membrane proteins remains difficult due to their hydrophobicity and their low natural abundance in cells. Furthermore, into established heterologous systems, these proteins are frequently only produced at very low levels, toxic
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