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安全信息

P4860

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

≥2.4 U/g

别名:

Alcalase® 2.4L, Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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About This Item

CAS号:
9014-01-1
EC 号:
3.4.21.62 (BRENDA, IUBMB)
EC 号:
232-752-2
MDL编号:
MFCD00132092
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形式

aqueous solution

质量水平

200

比活

≥2.4 U/g

储存温度

2-8°C

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相关类别

一般描述

蛋白水解酶具有催化、非催化和辅助结构域。蛋白水解酶大致分为内肽酶和外肽酶。根据功能可分为天冬氨酸、谷氨酸、半胱氨酸、苏氨酸、丝氨酸和金属蛋白酶。

应用

Sigma 公司的酶已被用于制备鸡（腿）骨蛋白水解产物，同时评估 ACE 抑制肽。在研究它们的抗肿瘤活性时，它还被用于水解蛋白核小球藻（Chlorella pyrenoidosa）蛋白质提取物。
蛋白酶是一种通过水解肽键来分解蛋白的酶。 蛋白酶用于降解蛋白质，研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。

生化/生理作用

碱性蛋白酶是丝氨酸型的内切蛋白酶。它具有广泛的底物特异性，可以水解蛋白质分子中的大多数肽键。它在 pH 6.5 和 8.5 之间有活性，最适温度为 60°C。该酶可用在洗涤剂配方中，以去除蛋白质污渍。
蛋白酶通过肽键的水解使蛋白质分解代谢。 蛋白酶被丝氨酸活性位点抑制剂灭活，例如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。

分析说明

用安森单位表示的活性

法律信息

Novozyme公司的产品
Alcalase is a registered trademark of Novozymes Corp.

警示用语：

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 2

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the composition of the solution in which P4860 Protease from Bacillus licheniformis is prepared?

    The composition of P4860 is 50% (w/w) glycerol, 41% (w/w) water, and 9% (w/w) enzyme.

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