biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PSR-45, monoclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Immunogen
磷酸丝氨酸与钉形贝血蓝蛋白(KLH)结合。
Biochem/physiol Actions
蛋白质磷酸化和脱磷酸化是改变真核细胞中蛋白质功能的基本信号传导机制。在正常组织中,磷酸化是一种罕见的遗传翻译后活动,但是磷酸化细胞蛋白质的丰度在多个激活过程后增加了数倍。被磷酸化的主要氨基酸是酪氨酸、丝氨酸或苏氨酸(pTyr/pSer/pThr),且每种氨基酸都有对其磷酸化的特异性激酶和对其脱磷酸化的特异性磷酸酶。针对于磷酸化残基的单克隆和多克隆抗体已经生成,并可用于分析和制备工具进行磷酸化细胞蛋白的鉴定、定量和免疫亲和分离。
通过 ELISA 和斑点印迹,该抗体以游离氨基酸形式或与载体(如 BSA 或 KLH)的缀合,与磷酸化丝氨酸特异性反应。与非磷酸化丝氨酸、磷酸苏氨酸、磷酸酪氨酸、AMP 或 ATP 无交叉反应。该抗体已用在免疫印迹中定位一些含磷酸丝氨酸蛋白质。由于识别位点的空间位阻,该抗体可能无法识别已知含有磷酸化丝氨酸的某些蛋白质。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
未找到合适的产品?
试试我们的产品选型工具.
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
法规信息
常规特殊物品
低风险生物材料
此项目有
An integrated assessment of histopathological changes of the enteric neuromuscular compartment in experimental colitis
Ippolito C, et al.
Journal of Cellular and Molecular Medicine, 19(2), 485-500 (2015)
Matteo Fornai et al.
The Journal of pharmacology and experimental therapeutics, 356(2), 434-444 (2015-11-20)
Parkinson's disease is frequently associated with gastrointestinal symptoms, mostly represented by constipation and defecatory dysfunctions. This study examined the impact of central dopaminergic denervation, induced by injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle, on distal colonic excitatory cholinergic
Mechanisms of specificity in protein phosphorylation
Ubersax JA and Ferrell Jr JE
Nature Reviews in Molecular and Cell Biology, 8(7), 530-530 (2007)
Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis
Kiran MS, et al.
Vascular Cell, 3(1), 6-6 (2011)
Ling Lai et al.
Journal of pineal research, 45(4), 476-488 (2008-08-19)
Melatonin, via its MT1 receptor, but not the MT2 receptor, can modulate the transcriptional activity of various nuclear receptors - estrogen receptor alpha (ERalpha) and retinoic acid receptor alpha (RARalpha), but not ERbeta- in MCF-7, T47D, and ZR-75-1 human breast
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P5747-100UL | 04061838032461 |
| P5747-200UL | 04061834392323 |
| P5747-25UL | 04061838454065 |
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持