P7012
胃蛋白酶 来源于猪胃粘膜
lyophilized powder, ≥2,500 units/mg protein (E1%/280)
别名:
胃液素 来源于猪胃, 胃蛋白酶 A
登录 查看组织和合同定价。
选择尺寸
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010127
EC Number:
232-629-3
NACRES:
NA.54
MDL number:
产品名称
胃蛋白酶 来源于猪胃粘膜, lyophilized powder, ≥2,500 units/mg protein (E1%/280)
form
lyophilized powder
specific activity
≥2,500 units/mg protein (E1%/280)
mol wt
35 kDa
color
off-white to yellow
solubility
deionized water: soluble 10 mg/mL
10 mM HCl: soluble 4 mg/mL (Cold)
UniProt accession no.
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
Gene Information
pig ... LOC396892(396892)
正在寻找类似产品? 访问 产品对比指南
Analysis Note
E1%/280=14.7
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
蛋白质由E1%/280测定
Application
来自Sigma的酶已被用于消化粗制小麦醇溶蛋白。它已与其他酶一起用于证明固定和酶促消化在使用石蜡包埋组织的免疫组织化学分析中的影响。它已被用于消化（在使用免疫过氧化物酶技术之前）以减少支气管组织切片的非特异性背景染色。该酶也被用于从IgG制备F(ab)2 片段。
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
胃蛋白酶是一种用于消化蛋白质的肽酶，常用于从抗体制备Fab片段。来自猪胃粘膜的胃蛋白酶已被用于水解小鼠的干燥宫颈样本。
Biochem/physiol Actions
优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
胃蛋白酶可水解肽键，而不是酰胺或酯键。 胃蛋白酶可水解在肽键任一侧具有芳香酸的肽。 含硫氨基酸与肽键越接近，则越易于水解。胃蛋白酶优先切割苯丙氨酸和亮氨酸的羧基侧以及谷氨酸残基的羧基侧。 切割胰岛素β链的Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe和Phe-Tyr键。
胃蛋白酶是胃产生的主要蛋白水解酶。它通过切割内部肽键来消化蛋白质。
胃蛋白酶是胃产生的主要蛋白水解酶。它通过切割内部肽键来消化蛋白质。
该酶不会切割缬氨酸、丙氨酸或甘氨酸键。Z-L-酪氨酰-L-苯丙氨酸、Z-L-谷氨酰-L-酪氨酸或Z-L-甲硫氨酰-L-酪氨酸可作为胃蛋白酶消化的底物。胃蛋白酶受到多种含苯丙氨酸的肽的抑制。
Other Notes
一个酶活性单位是指在使用血红蛋白作为底物的三氯乙酸（TCA）可溶性产物测量中，在37°C、pH 2.0条件下每分钟产生0.001的ΔA280 所需的酶量。（终体积 = 16 mL。光程 = 1cm。）
有关胃蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
Chiara Zanoni et al.
Journal of agricultural and food chemistry, 65(40), 8829-8838 (2017-09-22)
This study had the objective of preparing a hempseed protein hydrolysate and investigating its hypocholesterolemic properties. The hydrolysate was prepared treating a total protein extract with pepsin. Nano HPLC-ESI-MS/MS analysis permitted identifying in total 90 peptides belonging to 33 proteins.
S B Halstead et al.
The Journal of experimental medicine, 146(1), 201-217 (1977-07-01)
Cultured mononuclear peripheral blood leukocytes (PBL) from nonimmune human beings and monkeys are nonpermissive to dengue 2 virus (D2V) infection at multiplicities of infection of 0.001-0.1, but become permissive when non-neutralizing dengue antibody is added to medium. D2V infection occurred
A digestion technique for the reduction of background staining in the immunoperoxidase method.
M Reading
Journal of clinical pathology, 30(1), 88-90 (1977-01-01)
Charles P Read et al.
Reproduction (Cambridge, England), 134(2), 327-340 (2007-07-31)
Cervical remodeling during pregnancy and parturition is a single progressive process that can be loosely divided into four overlapping phases termed softening, ripening, dilation/labor, and post partum repair. Elucidating the molecular mechanisms that facilitate all phases of cervical remodeling is
P Kirkpatrick et al.
Journal of clinical pathology, 37(6), 639-644 (1984-06-01)
The effects of different fixatives and enzymatic digestion procedures on the immunohistochemical demonstration of fibronectin and laminin in paraffin embedded tissues have been compared. None of the fixatives tested enabled staining of these proteins without enzymatic digestion. No intracytoplasmic laminin
实验方案
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持