产品名称
植物分级蛋白提取试剂盒, Suitable for any plant species or tissue
usage
sufficient for 20 extractions
storage temp.
−20°C
Quality Level
General description
Sigma的植物分级蛋白提取试剂盒(Plant Fractionated Protein Extraction Kit)是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提亲水或疏水蛋白的定性样品，以帮助用户开展下游的蛋白组学应用。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤(APTP)。本试剂盒包含五类试剂：一份植物特异性的蛋白酶抑制剂、一份用于抽提亲水蛋白质的专用配方试剂，一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂。以及还原剂三丁基膦(TBP)和烷化试剂碘乙酰胺。这些试剂能够增强等电聚焦和2-D凝胶电泳的分离效果。蛋白酶抑制剂是作用于丝氨酸、半胱氨酸、天冬氨酸、金属蛋白酶与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。含有4-（2-氨基乙基）苯磺酰氟（AEBSF）、抑氨肽酶素、抑肽素A、E-64、亮抑酶肽和1,10-菲咯啉。
在去除了多酚、鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提水溶性蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
在去除了多酚、鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提水溶性蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
常规特殊物品
此项目有
M Ferro et al.
Electrophoresis, 21(16), 3517-3526 (2000-11-18)
As a complementary approach to genome projects, proteomic analyses have been set up to identify new gene products. One of the major challenges in proteomics concerns membrane proteins, especially the minor ones. A procedure based on the differential extraction of
Yinghui Ying et al.
Plant physiology, 173(1), 812-824 (2016-11-30)
Phosphate overaccumulator2 (PHO2) encodes a ubiquitin-conjugating E2 enzyme that is a major negative regulator of the inorganic phosphate (Pi)-starvation response-signaling pathway. A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between OsPHO2
Loomis, W.D.
Methods in Enzymology, 31, 528-545 null
Wenhao Yue et al.
The Plant journal : for cell and molecular biology, 90(6), 1040-1051 (2017-02-24)
Inorganic phosphate (Pi) transporters (PTs) play vital roles in Pi uptake and translocation in plants. Under Pi sufficient conditions, PTs are degraded to prevent excess Pi accumulation. The mechanisms targeting PTs for degradation are not fully elucidated. In this study
M Cilia et al.
Journal of biomolecular techniques : JBT, 20(4), 201-215 (2009-09-02)
Protein extraction methods can vary widely in reproducibility and in representation of the total proteome, yet there are limited data comparing protein isolation methods. The methodical comparison of protein isolation methods is the first critical step for proteomic studies. To
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