产品名称
过氧化物酶体分离试剂盒, isolate peroxisomes from tissues and cells
technique(s)
centrifugation: suitable
fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
过氧化物酶体分离试剂盒提供了使用碘克沙醇[OptiPrep™]的密度梯度离心法从动物组织和细胞质分离高度纯化的过氧化物酶体所需的一切试剂及详细实验方案。该试剂盒还可用于通过大鼠肝脏、大鼠肾脏、兔肝脏及HEK293和HepG2细胞制备过氧化物酶体。
Features and Benefits
- 专为研究规模应用而配制的提取试剂 - 有助于节省时间，最大限度减少浪费
- 可获得完整的功能性细胞器 - 所得的过氧化物酶体适于功能性研究、代谢检测、蛋白谱分析和疾病状态分析
- 兼容结构验证产品 - 可采用随附的细胞色素c还原酶检测试剂盒轻松进行完整性验证（货号：CY0100）
General description
分离所得的过氧化物酶体可用于研究脂质β-氧化、氨基酸代谢及醚链连接的甘油糖脂和胆汁酸的生物合成。
Other Notes
收到试剂盒后，蛋白酶抑制剂混合物（产品代码 P 8340）应储存在 –20°C 条件下，OptiPrep 密度梯度培养基（产品代码 O3028）应储存在室温下。
Legal Information
OptiPrep is a trademark of Serumwerk Bernburg AG
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
常规特殊物品
低风险生物材料
此项目有
Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
P B Lazarow et al.
Proceedings of the National Academy of Sciences of the United States of America, 73(6), 2043-2046 (1976-06-01)
Purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-CoA oxidizing system that uses O2 and NAD as electron acceptors. The system was detected by the ability of added palmitoyl-CoA to elicit O2 consumption, H2O2 production, and O2-dependent NAD reduction. The
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
商品
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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