Merck
CN

PEROX1

Sigma-Aldrich

过氧化物酶体分离试剂盒

isolate peroxisomes from tissues and cells

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NACRES:
NA.32

质量水平

运输

wet ice

储存温度

2-8°C

一般描述

分离所得的过氧化物酶体可用于研究脂质β-氧化、氨基酸代谢及醚链连接的甘油糖脂和胆汁酸的生物合成。

应用

过氧化物酶体分离试剂盒提供了使用碘克沙醇[OptiPrep]的密度梯度离心法从动物组织和细胞质分离高度纯化的过氧化物酶体所需的一切试剂及详细实验方案。该试剂盒还可用于通过大鼠肝脏、大鼠肾脏、兔肝脏及HEK293和HepG2细胞制备过氧化物酶体。

特点和优势

  • 专为研究规模应用而配制的提取试剂 - 有助于节省时间,最大限度减少浪费
  • 可获得完整的功能性细胞器 - 所得的过氧化物酶体适于功能性研究、代谢检测、蛋白谱分析和疾病状态分析
  • 兼容结构验证产品 - 可采用随附的细胞色素c还原酶检测试剂盒轻松进行完整性验证(货号:CY0100)

其他说明

收到试剂盒后,蛋白酶抑制剂混合物(产品代码 P 8340)应储存在 –20°C 条件下,OptiPrep 密度梯度培养基(产品代码 O3028)应储存在室温下。

法律信息

OptiPrep is a trademark of Serumwerk Bernburg AG

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书

象形图

Corrosion

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

分析证书(COA)

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示例

T1503
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批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

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  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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Dieanira Erudaitius et al.
Free radical biology & medicine, 120, 356-367 (2018-03-31)
The high extracellular hydrogen peroxide (H2O2) concentrations generated during pharmacological ascorbate (P-AscH-) therapy has been shown to exhibit a high flux into susceptible cancer cells leading to a decrease in clonogenic survival. It is hypothesized that the intracellular H2O2 concentration
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
K Burdett et al.
The Journal of biological chemistry, 266(19), 12201-12206 (1991-07-05)
Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which

商品

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

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