一般描述
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies. In immunohistochemistry (IHC), the most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves the accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.
应用
在免疫组化（IHC）中应用抗体之前，胃蛋白酶用于福尔马林固定石蜡包埋（FFPE）组织切片的蛋白水解消化。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
低风险生物材料
The effect of low doses of cadmium and zinc chloride on the Blood-Testis Barrier of Sprague Dawley rats.
Redondo A, et al.
arXiv (2012)
Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes.
Rwigemera A, et al.
Epigenetics & Chromatin, 10(1), 19-19 (2017)
Control of alveolar bone development, homeostasis, and socket healing by salt inducible kinases.
Tokavanich N, et al.
bioRxiv : the preprint server for biology, 2024-2009 (2024)
Comprehensive Assessment of the Virulence Factors sub 3, sub 6 and mcpA in the Zoonotic Dermatophyte Trichophyton benhamiae Using FISH and qPCR
Baumbach C M, et al.
Journal of fungi (Basel, Switzerland), 8(1), 24-24 (2021)
Codistribution of collagen type IV and laminin in liver fibrosis of elderly cadavers: immunohistochemical marker of perisinusoidal basement membrane formation.
Mak Ki M, et al.
The Anatomical Record, 296(6)A, 953-964 (2013)
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