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R6513

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

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别名:
RNAsea, RNase A, 核糖核酸 3′-嘧啶寡核苷酸水解酶, 核糖核酸酶 I, 胰核糖核酸酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.53

等级

for molecular biology

质量水平

形式

lyophilized

specific activity

≥70 Kunitz units/mg protein

分子量

13.7 kDa
~13,700

异质活性

Exonuclease and endonuclease, none detected
NICKase and DNase, none detected

储存温度

−20°C

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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此商品
R5503R5500R5000
form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

solid

specific activity

≥70 Kunitz units/mg protein

specific activity

50-100 Kunitz units/mg protein

specific activity

75-125 Kunitz units/mg protein

specific activity

>= 60 Kunitz units/mg protein

mol wt

13.7 kDa, ~13,700

mol wt

~13,700

mol wt

~13,700

mol wt

~13,700

foreign activity

Exonuclease and endonuclease, none detected, NICKase and DNase, none detected

foreign activity

protease, essentially free

foreign activity

protease, essentially free

foreign activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

RNA 酶 A 是一种内切核糖核酸酶,攻击嘧啶核苷酸的 3'OH磷酸。切割 pG-pG-pC-pA-pG 的序列,得到 pG-pG-pCp 和 A-pG。单链 RNA 表现出最高的活性。
RNase A是包含124个氨基酸的单链多肽,通过四个二硫键连接。与RNase B不同,RNase A不是糖蛋白。通过烷基化来酶活性位点中的His12或His119,可抑制RNase A。RNA酶A的激活剂包括钾盐和钠盐。
RNase A(核糖核酸酶A)是一种内切核糖核酸酶,在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端(例如,pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG)。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同,并非糖蛋白。核糖核酸酶不会水解DNA,因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外,RNase也被DNA竞争性抑制。

应用

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。
来自牛胰腺的核糖核酸酶A已用于:
  • 质粒DNA纯化
  • 在细胞核染色过程中,酶解双链RNA并避免染色
  • 免疫过氧化物酶和免疫荧光检测
  • 流式细胞分析前的细胞处理
  • 用作杂交后缓冲液组分,在原位杂交后洗涤FFPE组织切片
  • 在制备外膜蛋白(OMP)提取物期间处理细胞
  • 核糖核酸酶保护试验
  • 去除非特异性结合的RNA
  • 核糖核酸序列的分析
  • 水解蛋白质样品所含的RNA

特点和优势

我们高度稳定的核糖核酸酶A——RNase A,适合于RNA去除、RNA测序和DNA纯化。

分析说明

蛋白测定方法:E.

其他说明

RNase A的一个主要应用是从质粒DNA的制备中去除RNA。此类应用中,无DNase的RNase A的使用终浓度为10 μg/mL。

该RNase A产品不需要煮沸原液以灭活剩余的DNase,这有可能导致RNase沉淀,并可能导致酶活性损失。如果RNase溶液在中性pH下加热,就会发生沉淀。当在较低的pH值下加热时,由于存在蛋白质杂质,可能会发生一些沉淀。
RNase A的激活剂包括钾和钠盐。 RNase A可被烷基化的His12或His119所抑制。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the solubility of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?

    Stock solutions of RNase A can be made in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL.

  3. What is the shelf life of Product R6513, Ribonuclease A from bovine pancreas (RNase A)? 

    This product is stable for at least 3 years when stored properly at -20 °C.

  4. How are solutions of Product R6513, Ribonuclease A from bovine pancreas (RNase A), stored?

    RNase A solutions can be stored, aliquoted and frozen at -20 °C.

  5. Do I need to boil Product R6513, Ribonuclease A from bovine pancreas (RNase A), to inactivate residual DNases?

    Boiling of stock solutions to inactivate residual DNase is not necessary and may cause precipitation of RNase with loss of enzymatic activity. 

  6. What working concentration of Product R6513, Ribonuclease A from bovine pancreas (RNase A), do you suggest?

    We suggest using RNase A at a concentration of 10 ug/ml.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?

    The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Santella, Regina M., and Yu-Jing Zhang
DNA Fingerprinting: An Introduction (2011)
Rapid and irreversible CD4+ T-cell depletion induced by the highly pathogenic simian/human immunodeficiency virus SHIVDH12R is systemic and synchronous
Igarashi Tatsuhiko, et al.
Journal of Virology (2002)
Berg JM, Tymoczko JL, Stryer L.
BioChemistry: An Indian Journal (2002)
Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium in the same egg yolk
Chalghoumi, Raja, et al.
Poultry Science (2008)
Kento Kurata et al.
International journal of oncology, 49(6), 2303-2308 (2016-10-18)
Anaplastic thyroid cancer (ATC) is a rare malignancy that progresses extremely aggressively and often results in dismal prognosis. We investigated the efficacy of inhibiting the activated RAS/RAF/MEK pathway in ATC cells aiming to clarify the mechanism of effect and resistance.

实验方案

核糖核酸酶A(EC 3.1.27.5)的酶促测定

本实验方案可用于测定核糖核酸酶A(RNase A)的活性。

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity.

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