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Merck
CN

S4942

SYPRO ™ 红宝石蛋白凝胶染色剂

别名:

SYPRO™ dye, protein gel stain

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UNSPSC代码:
41105322
NACRES:
NA.32

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保质期

≥6 mo. (when stored at room temperature and protected from light)

质量水平

100

技术

protein staining: suitable

荧光

λex 280,450 nm; λem 610 nm

相关类别

一般描述

SYPRO Ruby 蛋白凝胶染色剂是一种即用型、超灵敏的发光染色剂，用于检测聚丙烯酰胺凝胶电泳 (PAGE) 分离的蛋白。该染色剂专为 2-D PAGE 设计，已被证明是 1-D PAGE 和等电聚焦 (IEF) 凝胶的极佳选择。SYPRO 红宝石蛋白凝胶染色的灵敏度与许多银染技术相当。但是，与银染不同的是，SYPRO Ruby 凝胶染色：
  • 使用简单的染色方案，无过度染色的可能性
  • 提供超过三个数量级的线性定量范围
  • 显示较少的蛋白质间变异性
  • 染色糖蛋白、脂蛋白、钙结合蛋白、纤维状蛋白和其他难染色蛋白
  • 不会染色外源性核酸
  • 不会干扰基于 Edman 测序或质谱的蛋白质后续分析

应用

SYPRO 红宝石蛋白凝胶染色剂用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 后的蛋白染色。

法律信息

SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

免责声明

避光保存。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCZ6821
MKCZ6125
MKCZ5142
MKCX8342
MKCX6305

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Christoph Schröder et al.
Methods in molecular biology (Clifton, N.J.), 785, 203-221 (2011-09-09)
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Filipe Natalio et al.
Cell and tissue research, 339(2), 429-436 (2009-12-17)
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations
Chronic hypoxia alters mitochondrial composition in human macrophages.
Fuhrmann DC, et al.
Biochimica et Biophysica Acta, 1834, 2750-2760 (2013)
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The Journal of general physiology, 154(10) (2022-09-03)
The β-myosin heavy chain expressed in ventricular myocardium and the myosin heavy chain (MyHC) in slow-twitch skeletal Musculus soleus (M. soleus) type-I fibers are both encoded by MYH7. Thus, these myosin molecules are deemed equivalent. However, some reports suggested variations
Saskia Hutten et al.
Cell reports, 33(12), 108538-108538 (2020-12-29)
Nuclear import receptors, also called importins, mediate nuclear import of proteins and chaperone aggregation-prone cargoes (e.g., neurodegeneration-linked RNA-binding proteins [RBPs]) in the cytoplasm. Importins were identified as modulators of cellular toxicity elicited by arginine-rich dipeptide repeat proteins (DPRs), an aberrant
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商品

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

蛋白电泳染色剂选择指南

为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。

实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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