S6072
Anti-STIM1 (N-terminal) 兔抗
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-GOK, Anti-Stromal interaction molecule I
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
20
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 90 kDa
species reactivity
rat, mouse, human
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells., indirect immunofluorescence: 5-10 μg/mL using human HeLa cells., western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... STIM1(6786)
mouse ... Stim1(20866)
rat ... Stim1(117086)
General description
STIM1 (stromal interaction molecule 1) is a conserved single-pass transmembrane protein, which is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 is modified by phosphorylation and N-linked glycosylation. STIM1 localized predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels.
Immunogen
synthetic peptide corresponding to amino acids 61-74 of human STIM1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
Application
Anti-STIM1 (N-terminal) antibody produced in rabbit has been used in:
- western blotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
STIM1 (stromal interaction molecule 1) is required for the activation of store-operated Ca2+ influx. Overexpression of STIM1 and Orai1 markedly increases the calcium release-activated calcium (CRAC) current (I-CRAC).
Physical form
Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
STIM1 is a Ca 2+ sensor that activates CRAC channels and migrates from the Ca 2+ store to the plasma membrane
Zhang SL, et al.
Nature, 437(7060), 902-902 (2005)
Inhibition of store-operated calcium entry attenuates MPP+-induced oxidative stress via preservation of mitochondrial function in PC12 cells: involvement of Homer1a
Li X, et al.
PLoS ONE, 8(12), e83638-e83638 (2013)
Alicia Sampieri et al.
Scientific reports, 8(1), 13252-13252 (2018-09-07)
The involvement of inositol trisphosphate receptor (IP3R) in modulating store-operated calcium entry (SOCE) was established many years ago. Nevertheless, the molecular mechanism responsible for this observation has not been elucidated to this date. In the present study we show that
Stephen Baine et al.
Journal of cellular and molecular medicine, 25(10), 4637-4648 (2021-03-24)
Heart failure (HF) is characterized by asymmetrical autonomic balance. Treatments to restore parasympathetic activity in human heart failure trials have shown beneficial effects. However, mechanisms of parasympathetic-mediated improvement in cardiac function remain unclear. The present study examined the effects and
Vivek Krishnan et al.
eLife, 11 (2022-02-12)
Peripheral coupling between the sarcoplasmic reticulum (SR) and plasma membrane (PM) forms signaling complexes that regulate the membrane potential and contractility of vascular smooth muscle cells (VSMCs). The mechanisms responsible for these membrane interactions are poorly understood. In many cells
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持