S6197
AntiSTIM1 (C-terminal) 兔抗
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
AntiGOK, AntiStromal interaction molecule 1
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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
16
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 90 kDa
species reactivity
human, rat, mouse
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells., indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells., western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... STIM1(6786)
mouse ... Stim1(20866)
rat ... Stim1(117086)
General description
Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels.
Immunogen
synthetic peptide corresponding to amino acids 657-683 of human STIM1, conjugated to KLH via an N-terminal residue. The corresponding sequence is identical in rat, mouse, bovine, monkey, and dog.
Application
Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in:
- western blot
- immunoprecipitation
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca2+ influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca2+ store depletion.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
SOCE induced calcium overload regulates autophagy in acute pancreatitis via calcineurin activation
Zhu ZD, et al.
Cell Death & Disease, 9(2), 50-50 (2018)
STIM1/Orai1-mediated store-operated Ca2+ entry: the tip of the iceberg
Giachini FR, et al.
Brazilian journal of medical and biological research, 44(11), 1080-1087 (2011)
Ana P Kutschat et al.
Cancer research, 81(11), 2943-2955 (2021-01-14)
Pancreatic ductal adenocarcinoma (PDAC) displays a dismal prognosis due to late diagnosis and high chemoresistance incidence. For advanced disease stages or patients with comorbidities, treatment options are limited to gemcitabine alone or in combination with other drugs. While gemcitabine resistance
Store-operated Ca2+ entry mediated by Orai1 and TRPC1 participates to insulin secretion in rat beta-cells
Sabourin J, et al.
The Journal of Biological Chemistry, 290(51), 30530-30539 (2015)
Cooperative Binding of Stromal Interaction Molecule 1 (STIM1) to the N and C Termini of Calcium Release-activated Calcium Modulator 1 (Orai1)
Palty R, et al.
The Journal of Biological Chemistry, 291(1), 334-341 (2016)
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