SAB4200330
Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit
IgG fraction of antiserum
别名:
Anti-CD247, Anti-CD3H, Anti-CD3Q, Anti-CD3Z, Anti-T cell receptor T3 zeta chain, Anti-T3Z, Anti-TCRK, Anti-Tcrz
产品名称
Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit, IgG fraction of antiserum
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
technique(s)
immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
western blot: 1:1,000-1:2,000 using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr72)
Quality Level
Gene Information
mouse ... Cd247(12503)
Application
Anti-phospho-CD3ζ (pTyr72) antibody has been used in
- immunoblotting
- immunoprecipitation
- western blotting
Biochem/physiol Actions
T cell antigen receptor ζchain (CD3ζ) functions as a key signal transduction component of the T cell antigen receptor (TCR) complex. The encoded protein facilitates optimal effector T-cell function by enhancing receptor expression and signaling. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE). Reduced expression of the gene has been observed in T-cells of cancer, lupus and chronic infectious diseases such as leprosy and tuberculosis patients. CD3ζ serves as a potent biomarker for determining progression and severity in patients with type 2 diabetes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
T cell antigen receptor ζ chain (CD3ζ), also known as CD247 gene, spanning 88kb of genomic DNA, is mapped to human chromosome 1q24.2.
The T cell antigen receptor-CD3 (TCR-CD3) multimeric complex is composed of TCRαβ, CD3γε, CD3δε and ζ ζ dimers. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.
Immunogen
synthetic phosphopeptide corresponding to residues surrounding Tyr72 of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by a single amino acid in human.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
An immunoproteomic approach to characterize the CAR interactome and signalosome
Ramello M C, et al.
Science Signaling, 12(568), eaap9777-eaap9777 (2019)
Crippling of CD3-zeta ITAMs does not impair T cell receptor signaling
Ardouin L, et al.
Immunity, 10(4), 409-420 (1999)
Regulation of T Cell Receptor CD3? Chain Expression byL-Arginine
Rodriguez PC
The Journal of Biological Chemistry, 277, 21123-21129 (2002)
CD247, a Novel T Cell?Derived Diagnostic and Prognostic Biomarker for Detecting Disease Progression and Severity in Patients With Type 2 Diabetes
Eldor R
Diabetes Care, 38, 113-118 (2015)
Genetic Association of CD247 (CD3?) with SLE in a Large-Scale Multiethnic Study
Martins M
Genes and Immunity, 16, 142-150 (2015)
相关内容
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