产品名称
Anti-Nestin (C-terminal region) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~300 kDa
species reactivity
rat, human, mouse
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells.
western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)
Application
Anti-Nestin (C-terminal region) antibody has been used in
- immunofluorescence
- Proximity ligation assay
- immunohistofluorescence staining
- immunofluorescence labeling
- immunoblotting
Anti-Nestin (C-terminal region) antibody produced in rabbit has been used in western blotting and immunostaining.
Biochem/physiol Actions
Nestin (NES) functions as a modulator of proteins which are involved in cell growth. It also interacts with other proteins of the intermediate filament. NES has been studied as a marker of neural stem cells.
Nestin expression is down-regulated and replaced by the expression of other intermediate filament proteins such as neurofilament and Glial fibrillary acidic protein (GFAP). Nestin expression is re-induced in the adult organism during pathological conditions, such as formation of glial scar after injury to the central nervous system (CNS) and in certain types of CNS tumors. Nestin has been shown to play an important role in the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis and is a potential target for Cyclin-dependent kinase 5 (cdk5) and p35 during development.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Nestin (NES) is a class VI cytoplasmic protein which is expressed in the intermediate filaments. It has a molecular weight of 177.4kDa. The gene encoding it is localized on human chromosome 1q23.1 and has four exons.
Nestin is expressed during early stages of development in the central nervous system (CNS) and in myogenesis. Nestin is found in neurogenic progenitor cells (NPCs) and has been used as a marker for neural progenitor cells.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminal region of human nestin, conjugated to KLH. The sequence is highly conserved (72% identity) in rat and mouse nestin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Journal of Cellular Physiology (2019)
Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats.
Fang M
BMC Neuroscience (2015)
Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis
Chou Y, et al.
Molecular Biology of the Cell, 14(4), 1468-1478 (2003)
Nestin expression in hair follicle sheath progenitor cells
Li L, et al.
Proceedings of the National Academy of Sciences of the USA, 100(17), 9958-9961 (2003)
Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats
Fang M, et al.
BMC Neuroscience, 16(1), 84-84 (2015)
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