SAB4200399
Anti-WIPI-2 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WIPI2, WD repeat domain, phosphoinositide interacting 2
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
western blot
western blot
Species reactivity:
rat, mouse, human
Citations:
7
Technique(s):
immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.
western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
Uniprot accession no.:
产品名称
Anti-WIPI-2 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~49 kDa
species reactivity
rat, mouse, human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.
western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... WIPI2(26100)
mouse ... Wipi2(74781)
rat ... Wipi2(288498)
Application
Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2.
WIPI-2 (WD repeat domain, phosphoinositide-interacting 2) plays a vital role in the transformation of omegasomes into autophagosomes. The above protein functions as a mammalian effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI2 aids recombinant capsid protein VP1 (rVP1) in upregulating autophagy, MAPK1 (Mitogen-activated protein kinase 1)/3 phosphorylation and matrix metallopeptidase 9 (MMP-9) activity, which are required for macrophage migration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif.
WD repeat domain, phosphoinositide-interacting 2 (WIPI-2) belongs to the WIPI protein family. It is localized on the autophagy membrane and plasma membrane. Apart from this, it is also present on the membranes near the Golgi cisternae. WIPI2 is a mammalian ortholog of Atg18, which is ubiquitously expressed in a variety of cell lines.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Polson HE
Autophagy (2010)
Recombinant protein rVP1 upregulates BECN1-independent autophagy, MAPK1/3 phosphorylation and MMP9 activity via WIPI1/WIPI2 to promote macrophage migration.
Liao CC
Autophagy (2013)
Melanie Haas Kucherlapati
Oncotarget, 10(65), 6913-6933 (2019-12-21)
Genes of the pre-replication, pre-initiation and replisome complexes duplicate the genome from many sites once in a normal cell cycle. This study examines complex components in lung adenocarcinoma (LUAD) closely, correlating changes in the genome and transcriptome with proliferation and
Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.
Proikas-Cezanne T
Journal of Cellular and Molecular Medicine (2011)
Wei Wan et al.
The EMBO journal, 42(8), e112387-e112387 (2023-03-07)
The cGAS-STING pathway plays an important role in host defense by sensing pathogen DNA, inducing type I IFNs, and initiating autophagy. However, the molecular mechanism of autophagosome formation in cGAS-STING pathway-induced autophagy is still unclear. Here, we report that STING
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